Institute of Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Department of Oral Surgery, University Hospital Düsseldorf, Düsseldorf, Germany.
PLoS One. 2022 Jul 8;17(7):e0270962. doi: 10.1371/journal.pone.0270962. eCollection 2022.
To characterize a potential pathogenic role of Mycoplasma salivarium and bacterial co-detection patterns on different implant augmentation types.
36 patients were non-randomly assigned to autogenous lateral alveolar ridge augmentation with either cortical autogenous bone blocks, or healthy autogenous tooth roots or non-preservable teeth. Mucosal inflammation was assessed by probing pocket depth (PD) at all sampling sites and by bleeding on probing (BOP) in a subset of sampling sites, and standardized biofilm samples were obtained from the submucosal peri-implant sulcus and sulcus of a contralateral tooth at two times (t1 after implant placement; t2 after six months). Seven bacterial species were quantified using Taqman PCR.
Mucosal inflammation did not differ between augmentation groups, but peri-implant sulci showed increased abundance of M. salivarium after augmentation with autogenous tooth roots lasting for at least six months (t1 p = 0.05, t2 p = 0.011). In M. salivarium-positive samples, Tannerella forsythia was correlated with PD (R = 0.25, p = 0.035) This correlation was not observed in M. salivarium-negative samples. Compared to all other samples, PD was deeper in co-detection (i.e., simultaneous M. salivarium and T. forsythia) positive samples (p = 0.022). No association of single or co-detection of bacteria with BOP was observed.
Presence of M. salivarium in peri-implant sulci varies with augmentation method and is associated with increased PD but not BOP. A potential causal role of M. salivarium in inflammation through a mechanism involving co-presence of T. forsythia requires further study.
描述口腔支原体和细菌共同检出模式在不同种植体增强类型中的潜在致病作用。
36 名患者被非随机分配到自体外侧牙槽嵴增强，增强材料为皮质自体骨块、健康自体牙根或不可保存牙齿。所有采样部位均通过探测袋深度（PD）和部分采样部位的探测出血（BOP）评估黏膜炎症，并在两次（植入后 t1；植入后 6 个月 t2）从黏膜下种植体周围沟和对侧牙齿的沟中获得标准化生物膜样本。使用 Taqman PCR 定量 7 种细菌。
增强组之间黏膜炎症无差异，但在使用自体牙根增强后至少 6 个月，种植体周围沟中 M. salivarium 的丰度增加（t1 p = 0.05，t2 p = 0.011）。在 M. salivarium 阳性样本中，Tannerella forsythia 与 PD 相关（R = 0.25，p = 0.035），在 M. salivarium 阴性样本中未观察到这种相关性。与所有其他样本相比，共同检出（即同时存在 M. salivarium 和 T. forsythia）阳性样本的 PD 更深（p = 0.022）。未观察到单一细菌或共同检出细菌与 BOP 之间存在关联。
种植体周围沟中 M. salivarium 的存在随增强方法而异，与 PD 增加相关，但与 BOP 无关。M. salivarium 通过涉及 T. forsythia 共同存在的机制在炎症中发挥潜在因果作用，需要进一步研究。
