发展快速低温辅助改良 QuEChERS 法，同时测定动物脂肪中的 107 种农药及其相关代谢物。
Development of rapid low temperature assistant modified QuEChERS method for simultaneous determination of 107 pesticides and relevant metabolites in animal lipid.
机构信息
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China.
Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China.
出版信息
Food Chem. 2022 Nov 30;395:133606. doi: 10.1016/j.foodchem.2022.133606. Epub 2022 Jun 30.
A modified QuEChERS LC-MS/MS method was developed for 107 pesticides analysis in animal lipids such as pork lard, mutton tallow, chicken oil and butter. The challenges for high fat matrices clean up were studied in details by optimizing dispersive purification adsorbents coupled with rapid low temperature assistant methods. The method validation was carried out using pork lard and further appplied to other matrices by testing their recoveries. Good linearities were obtained with correlation coefficients greater than 0.99. Sensitive LOQs ranged from 5.0-50.0 μg kg. Both inter-day and intra-day precisions were lower than 20% indicating the good precision and accuracy of this method. The method applied to four animal lipids with 93%∼100% of analytes revealed satisfactory recoveries (ranged from 70% to 120%) and RSD (≤20%) at 10 µg kgand 50 µg kg spiking levels respectively related to the matrix.
建立了一种改良的 QuEChERS LC-MS/MS 方法，用于分析猪肉脂、羊肉脂、鸡油和黄油等动物脂肪中的 107 种农药。通过优化分散净化吸附剂并结合快速低温辅助方法，详细研究了高脂肪基质净化的挑战。该方法通过使用猪油进行验证，并通过测试回收率将其应用于其他基质。良好的线性相关系数大于 0.99。灵敏度 LOQs 范围为 5.0-50.0 μg kg。日内和日间精密度均低于 20%，表明该方法具有良好的精密度和准确性。该方法应用于四种动物脂肪，在 10 μg kg 和 50 μg kg 加标水平下，与基质相关的分析物回收率为 93%∼100%，RSD（≤20%）。
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