Vos J J, Huisman J G, Modderman P W, von dem Borne A E
Br J Haematol. 1987 Apr;65(4):447-50. doi: 10.1111/j.1365-2141.1987.tb04148.x.
A radioimmunoassay (RIA) for the detection of platelets and platelet fragments was developed. A sandwich of two monoclonal antibodies directed against the platelet-specific glycoprotein complex IIb-IIIa (GP IIb-IIIa) was used in this assay. A discontinuous 7.5-20% (v/v) albumin gradient was applied to separate platelets and their fragments of various sizes. In platelet suspensions fractionated in this way, we observed that particles smaller than normal platelets still carried the GP IIb-IIIa antigens. This procedure enabled us to detect platelet-derived particles in platelet-rich plasma from thrombocytopenic patients.
开发了一种用于检测血小板和血小板碎片的放射免疫测定法（RIA）。该测定法使用了针对血小板特异性糖蛋白复合物IIb-IIIa（GP IIb-IIIa）的两种单克隆抗体组成的夹心结构。应用不连续的7.5-20%（v/v）白蛋白梯度来分离不同大小的血小板及其碎片。在以这种方式分级分离的血小板悬液中，我们观察到小于正常血小板的颗粒仍携带GP IIb-IIIa抗原。该方法使我们能够在血小板减少症患者的富含血小板血浆中检测到血小板衍生颗粒。
