State Key Laboratory of Metastable Materials Science and Technology, Key Laboratory of Applied Chemistry, Nano-biotechnology Key Lab of Hebei Province, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, Yanshan University, Qinhuangdao 066004, China.
Hebei Key Laboratory for Chronic Diseases, School of Basic Medicine, North China University of Science and Technology, Tangshan 063210, China.
Colloids Surf B Biointerfaces. 2022 Sep;217:112681. doi: 10.1016/j.colsurfb.2022.112681. Epub 2022 Jun 30.
The development of stealth and effective antitumor nanodrugs has been drawing great attention. Herein, generation five poly(amide amine) dendrimer (G5 PAMAM) was modified by zwitterionic material carboxybetaine methacrylamide (CBMAA) on its surface to prepare zwitterionic dendrimer (G5-CBMAA). The results showed that G5-CBMAA had the longest blood circulation time due to its thickest zwitterionic layer, and its residual rate after injection into mice at 2 and 12 h was as high as 47.22 % and 14.37 %, respectively. Nanodrug G5-CBMAA-ICG was prepared by containing indocyanine green (ICG) in the cavity of G5-CBMAA. G5-CBMAA-ICG had better tumor targeting ability and antitumor effect than free ICG in mice after laser irradiation, and the tumor inhibition rate was 96.6 % after 14 days' treatment. The prepared G5-CBMAA-ICG has great potential applications in the field of antitumor by phototherapy.
具有隐身和高效抗肿瘤作用的纳米药物的研发受到广泛关注。本研究通过在第五代聚酰胺-胺树枝状大分子（G5 PAMAM）表面修饰两性离子材料羧酸甜菜碱甲基丙烯酰胺（CBMAA），制备了两性离子树枝状大分子（G5-CBMAA）。结果表明，由于其最厚的两性离子层，G5-CBMAA 具有最长的血液循环时间，其在注射入小鼠 2 和 12 小时后的残留率分别高达 47.22%和 14.37%。通过将吲哚菁绿（ICG）包封在 G5-CBMAA 的空腔中，制备了纳米药物 G5-CBMAA-ICG。在激光照射下，G5-CBMAA-ICG 在小鼠体内具有比游离 ICG 更好的肿瘤靶向能力和抗肿瘤效果，治疗 14 天后肿瘤抑制率达到 96.6%。通过光疗，所制备的 G5-CBMAA-ICG 在抗肿瘤领域具有巨大的应用潜力。
