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rhBMP6 复合自体血凝胶与合成陶瓷诱导兔长期脊柱后路融合

Long-term posterolateral spinal fusion in rabbits induced by rhBMP6 applied in autologous blood coagulum with synthetic ceramics.

机构信息

Laboratory for Mineralized Tissues, Center for Translational and Clinical Research, School of Medicine, University of Zagreb, Salata 11, 10000, Zagreb, Croatia.

Scientific Center of Excellence for Reproductive and Regenerative Medicine, Zagreb, Croatia.

出版信息

Sci Rep. 2022 Jul 8;12(1):11649. doi: 10.1038/s41598-022-14931-2.

DOI:10.1038/s41598-022-14931-2
PMID:35803983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9270325/
Abstract

Autologous bone graft substitute (ABGS) containing rhBMP6 in autologous blood coagulum (Osteogrow) is a novel therapeutic solution for bone regeneration. This study is aimed to investigate the long-term outcome of ABGS with synthetic ceramics (Osteogrow-C) in rabbit posterolateral spinal fusion (PLF) model. Osteogrow-C implants were implanted bilaterally between rabbit lumbar transverse processes. We compared the outcome following implantation of ABGS with ceramic particles of different chemical composition (TCP and biphasic ceramics containing both TCP and HA) and size (500-1700 µm and 74-420 µm). Outcome was analyzed after 14 and 27 weeks by microCT, histology, and biomechanical analyses. Successful bilateral spinal fusion was observed in all animals at the end of observation period. Chemical composition of ceramic particles has impact on the PLF outcome via resorption of TCP ceramics, while ceramics containing HA were only partially resorbed. Moreover, persistence of ceramic particles subsequently resulted with an increased bone volume in implants with small particles containing high proportion of HA. ABGS (rhBMP6/ABC) with various synthetic ceramic particles promoted spinal fusion in rabbits. This is the first presentation of BMP-mediated ectopic bone formation in rabbit PLF model with radiological, histological, and biomechanical features over a time course of up to 27 weeks.

摘要

自体骨移植替代物（ABGS）含 rhBMP6 于自体血凝块（Osteogrow）中是一种新的骨再生治疗方案。本研究旨在探讨含有合成陶瓷（Osteogrow-C）的 ABGS 在兔后外侧脊柱融合（PLF）模型中的长期疗效。Osteogrow-C 植入物双侧植入兔腰椎横突之间。我们比较了不同化学成分（TCP 和包含 TCP 和 HA 的双相陶瓷）和大小（500-1700µm 和 74-420µm）的陶瓷颗粒植入物的疗效。在 14 和 27 周时通过 microCT、组织学和生物力学分析评估结果。在观察期末，所有动物均成功进行双侧脊柱融合。陶瓷颗粒的化学成分通过 TCP 陶瓷的吸收对 PLF 结果产生影响，而含有 HA 的陶瓷仅部分吸收。此外，陶瓷颗粒的持续存在导致含有高比例 HA 的小颗粒植入物中的骨体积增加。含各种合成陶瓷颗粒的 ABGS（rhBMP6/ABC）促进了兔的脊柱融合。这是首次在兔 PLF 模型中展示 BMP 介导的异位骨形成的影像学、组织学和生物力学特征，时间长达 27 周。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/2c49c2077dcd/41598_2022_14931_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/8680ef6079ce/41598_2022_14931_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/c117e803b497/41598_2022_14931_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/10a4a1322d07/41598_2022_14931_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/867e6c5cfee5/41598_2022_14931_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/d6dff3a70a2a/41598_2022_14931_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/fdd8b94cd9fb/41598_2022_14931_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/06de4ab5fc93/41598_2022_14931_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/2c49c2077dcd/41598_2022_14931_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/8680ef6079ce/41598_2022_14931_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/c117e803b497/41598_2022_14931_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/10a4a1322d07/41598_2022_14931_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/867e6c5cfee5/41598_2022_14931_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/d6dff3a70a2a/41598_2022_14931_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/fdd8b94cd9fb/41598_2022_14931_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/06de4ab5fc93/41598_2022_14931_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcb5/9270325/2c49c2077dcd/41598_2022_14931_Fig8_HTML.jpg

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