Leake R D, Ervin M G, Ross M G, Stegner H, Fisher D A
Biol Neonate. 1987;51(4):204-11. doi: 10.1159/000242653.
Blood samples (4 ml) for plasma arginine vasopressin (AVP) measurements were obtained at 3- to 4-hour intervals under basal conditions for 1-2 days from 5 date-bred ewes with chronic maternal and fetal vascular catheters. In addition, 6 chronically catheterized ewes were infused with 2 liters of 0.45% NaCl over 30 min. Fetal and maternal blood samples were obtained before and after the infusion period for measurement of plasma osmolality and AVP concentrations. In the first study, maternal and fetal plasma AVP levels correlated significantly (p less than 0.01, by linear regression analysis) under basal conditions. In the second study, baseline mean (+/- SEM) plasma osmolality was similar for pregnant ewes and fetuses (303 +/- 3.1 and 302 +/- 2.4 mosm/kg, respectively). There was a significant (each, p less than 0.01 by paired t test) decrease from baseline in maternal and fetal osmolality during the 30 min after completion of the hypotonic saline (to 292 +/- 4.7 and 296 +/- 2.4 mosm/kg, respectively). Fetal plasma AVP levels decreased 17 +/- 6% by 30 min following the completion of water loading (1.7 +/- 0.07 to 1.4 +/- 0.16 microU/ml; p less than 0.05). Maternal plasma AVP levels decreased 16 +/- 4% by 30 min after completion of infusion (1.6 +/- 0.14 to 1.38 +/- 0.6 microU/ml; p less than 0.05). These results indicate that maternal and fetal plasma AVP levels correlate under basal conditions and that maternal water loading, which significantly decreases fetal plasma osmolality, significantly suppresses fetal plasma AVP concentrations.
在基础条件下，每隔3至4小时从5只植入慢性母体和胎儿血管导管的经同期发情处理的母羊采集4毫升血液样本，用于测量血浆精氨酸加压素（AVP），持续1至2天。此外，给6只慢性插管母羊在30分钟内输注2升0.45%氯化钠溶液。在输注前后采集胎儿和母体血液样本，用于测量血浆渗透压和AVP浓度。在第一项研究中，基础条件下母体和胎儿血浆AVP水平显著相关（线性回归分析，p<0.01）。在第二项研究中，怀孕母羊和胎儿的基线平均（±SEM）血浆渗透压相似（分别为303±3.1和302±2.4 mOsm/kg）。在低渗盐水输注完成后的30分钟内，母体和胎儿的渗透压与基线相比均显著降低（配对t检验，各p<0.01；分别降至292±4.7和296±2.4 mOsm/kg）。在水负荷完成后30分钟，胎儿血浆AVP水平下降17±6%（从1.7±0.07降至1.4±0.16 μU/ml；p<0.05）。输注完成后30分钟，母体血浆AVP水平下降16±4%（从1.6±0.14降至1.38±0.6 μU/ml；p<0.05）。这些结果表明，基础条件下母体和胎儿血浆AVP水平相关，且母体水负荷显著降低胎儿血浆渗透压，同时显著抑制胎儿血浆AVP浓度。
