Hauschild Philippa, Vogel Rudi F, Hilgarth Maik
Lehrstuhl Technische Mikrobiologie, Technische Universität München, Gregor-Mendel-Straße 4, 85354, Freising, Germany.
Arch Microbiol. 2022 Jul 8;204(8):467. doi: 10.1007/s00203-022-03059-6.
This study investigated the impact of Brochothrix (B.) thermosphacta and Pseudomonas (Ps.) fragi on the transcriptomes of Photobacterium (P.) phosphoreum and P. carnosum on chicken meat under modified atmosphere (MA) and air atmosphere (AA). P. phosphoreum TMW2.2103 responded to MA with a reduced transcript number related to cell division and an enhanced number related to oxidative stress. Concomitantly, the analysis revealed upregulation of fermentation and downregulation of respiration. It predicts enhanced substrate competition in presence of co-contaminants/MA. In contrast, the strain upregulated the respiration in AA, supposably due to improved substrate accessibility in this situation. For P. carnosum TMW2.2149 the respiration was downregulated, and the pyruvate metabolism upregulated under MA. MA/co-contaminant resulted in multiple upregulated metabolic routes. Conversely, AA/co-contaminant resulted only in minor regulations, showing inability to cope with fast growing competitors. Observations reveal different strategies of photobacteria to react to co-contaminants on meat.
本研究调查了嗜热栖热丝菌（B. thermosphacta）和脆弱假单胞菌（Ps. fragi）对鸡肉中磷发光杆菌（P. phosphoreum）和肉发光杆菌（P. carnosum）在气调包装（MA）和空气包装（AA）条件下转录组的影响。磷发光杆菌TMW2.2103对气调包装的响应是与细胞分裂相关的转录本数量减少，与氧化应激相关的转录本数量增加。同时，分析显示发酵上调，呼吸下调。这预示着在存在共污染物/气调包装的情况下底物竞争增强。相比之下，该菌株在空气包装中上调了呼吸作用，推测是由于这种情况下底物可及性提高。对于肉发光杆菌TMW2.2149，在气调包装下呼吸作用下调，丙酮酸代谢上调。气调包装/共污染物导致多种代谢途径上调。相反，空气包装/共污染物仅导致轻微的调节，表明无法应对快速生长的竞争者。观察结果揭示了发光细菌对肉中共污染物作出反应的不同策略。
