Nićetin Milica, Pezo Lato, Pergal Marija, Lončar Biljana, Filipović Vladimir, Knežević Violeta, Demir Hande, Filipović Jelena, Manojlović Dragan
Faculty of Technology Novi Sad, University of Novi Sad, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
Institute of General and Physical Chemistry, University of Belgrade, Studentski Trg. 12-16, 11000 Beograd, Serbia.
Foods. 2022 Jun 30;11(13):1945. doi: 10.3390/foods11131945.
The osmotic dehydration (OD) of celery root in sugar beet molasses was studied at three temperatures (20, 35, and 50 °C) and three immersion periods (1, 3, and 5 h) in order to examine the changes in antioxidant potential and phenolic profile of celery root throughout the process. The antioxidant capacity (AOC) of dehydrated samples was evaluated by spectrophotometric and polarographic assays, the total phenolic content by the Folin-Ciocalteu method, and the individual phenolic compounds by HPLC-DAD. As a result of OD in molasses, the AOC and phenols content in samples increased proportionally to the augmentation of temperature and the immersion time. Vanillic acid, syringic acid, and catechin were detected in dehydrated samples as a result of transfer from molasses. Compared to fresh celery root, the content of identified phenols in osmodehydrated samples was improved from 1.5 to 6.2 times. Strong correlations between applied assays were obtained, except for the DPPH. Based on the correlation analysis chlorogenic acid, gallic acid, chrysin, catechin, and kaempferol showed the greatest contribution to the overall AOC of osmodehydrated celery root. Molasses, an agro-industrial waste from sugar production, could be valorized as a valuable osmotic solution.
为了研究整个过程中芹菜根抗氧化能力和酚类物质组成的变化，在三个温度（20、35和50℃）和三个浸渍时间（1、3和5小时）下对芹菜根在甜菜糖蜜中的渗透脱水（OD）进行了研究。通过分光光度法和极谱法测定脱水样品的抗氧化能力（AOC），采用福林-酚法测定总酚含量，通过高效液相色谱-二极管阵列检测器（HPLC-DAD）测定单个酚类化合物。由于在糖蜜中进行渗透脱水，样品中的AOC和酚类物质含量随温度升高和浸渍时间延长而呈比例增加。由于糖蜜中的酚类物质转移到脱水样品中，检测到了香草酸、丁香酸和儿茶素。与新鲜芹菜根相比，渗透脱水样品中鉴定出的酚类物质含量提高了1.5至6.2倍。除了DPPH外，所采用的检测方法之间存在很强的相关性。基于相关性分析，绿原酸、没食子酸、白杨素、儿茶素和山奈酚对渗透脱水芹菜根的总体AOC贡献最大。糖蜜是制糖工业产生的一种农业废弃物，可以作为一种有价值的渗透溶液加以利用。
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