Giromini Carlotta, Baldi Antonella, Rebucci Raffaella, Lanzoni Davide, Policardi Martina, Sundaram Tamil Selvi, Purup Stig
Department of Veterinary and Animal Science, Università Degli Studi di Milano, Via dell'Università 6, 29600 Lodi, Italy.
CRC, Innovation for Well-Being and Environment, Università Degli Studi di Milano, 20122 Milano, Italy.
Foods. 2022 Jul 5;11(13):1983. doi: 10.3390/foods11131983.
Short chain fatty acids (SCFAs), especially butyrate (BUT), are known to promote intestinal health, but their role in the protection of intestinal barrier integrity is poorly characterized. The aim of the study was to set up an in vitro model of human colon epithelium using HT29-MTX-E12 cells to delineate the potential role of SCFAs under stress conditions. Accordingly, the HT29-MTX-E12 cells were differentiated for 42 days and subsequently exposed to dextran sulphate sodium (DSS). Further, the effects of BUT or its mixture with acetate and propionate (SCFAs-MIX) were tested to study proliferation, epithelial integrity and mucus production. The results showed that the concentration of 10% DSS for 24 h decreased the TEER about 50% compared to the control in HT29-MTX-E12 cells. The pre-treatment on HT29-MTX-E12 cells with BUT or SCFAs-MIX at specific concentrations significantly (p < 0.05) reduced the DSS-induced damage on epithelial cell integrity and permeability. Further, the treatment with specific concentrations of BUT and SCFAs-MIX for 24 h significantly promoted ZO-1, MUC2 and MUC5AC mRNA expression (p < 0.005). The present study demonstrated the suitability of HT29-MTX-E12 cells treated with DSS as an in vitro stress model of inflammatory bowel disease, which enabled us to understand the effect of bioactive SCFAs on the intestinal barrier.
短链脂肪酸（SCFAs），尤其是丁酸盐（BUT），已知可促进肠道健康，但其在保护肠道屏障完整性方面的作用尚不清楚。本研究的目的是建立一种使用HT29-MTX-E12细胞的人结肠上皮体外模型，以阐明应激条件下SCFAs的潜在作用。因此，将HT29-MTX-E12细胞分化42天，随后暴露于硫酸葡聚糖钠（DSS）。此外，测试了丁酸盐或其与乙酸盐和丙酸盐的混合物（SCFAs-MIX）对细胞增殖、上皮完整性和黏液产生的影响。结果表明，在HT29-MTX-E12细胞中，与对照相比，10% DSS浓度处理24小时可使跨上皮电阻（TEER）降低约50%。用特定浓度的丁酸盐或SCFAs-MIX对HT29-MTX-E12细胞进行预处理可显著（p < 0.05）减少DSS诱导的上皮细胞完整性和通透性损伤。此外，用特定浓度的丁酸盐和SCFAs-MIX处理24小时可显著促进紧密连接蛋白1（ZO-1）、黏蛋白2（MUC2）和黏蛋白5AC（MUC5AC）的mRNA表达（p < 0.005）。本研究证明了用DSS处理的HT29-MTX-E12细胞作为炎症性肠病体外应激模型的适用性，这使我们能够了解生物活性SCFAs对肠道屏障的影响。
