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超声处理对膜片悬浮液中微分吸收平坦化影响的理论分析。

A theoretical analysis of the effects of sonication on differential absorption flattening in suspensions of membrane sheets.

作者信息

Teeters C L, Eccles J, Wallace B A

出版信息

Biophys J. 1987 Apr;51(4):527-32. doi: 10.1016/S0006-3495(87)83378-7.

DOI:10.1016/S0006-3495(87)83378-7
PMID:3580481
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1329925/
Abstract

A theoretical analysis is presented for the flattening of absorption and circular dichroic spectra of suspensions of membrane sheets containing proteins. Equations are presented to describe the dependence of this artifact on the size of the sheets. Values for the flattening coefficients QA and QB are calculated both as a function of absorptivity and sheet size. These studies show that sonication in an inadequate procedure for eliminating flattening in these samples.

摘要

本文对含有蛋白质的膜片悬浮液的吸收光谱和圆二色光谱的扁平化进行了理论分析。给出了描述这种假象与膜片大小相关性的方程。计算了扁平化系数QA和QB作为吸光率和膜片大小函数的值。这些研究表明，超声处理并非消除这些样品中扁平化的合适方法。

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A theoretical analysis of the effects of sonication on differential absorption flattening in suspensions of membrane sheets.超声处理对膜片悬浮液中微分吸收平坦化影响的理论分析。
Biophys J. 1987 Apr;51(4):527-32. doi: 10.1016/S0006-3495(87)83378-7.
2
Circular dichroism analyses of membrane proteins: an examination of differential light scattering and absorption flattening effects in large membrane vesicles and membrane sheets.膜蛋白的圆二色性分析：对大膜泡和膜片层中差分光散射和吸收平坦化效应的研究。
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Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilamellar vesicles.在小单层囊泡的圆二色光谱中，差分光散射和吸收平坦化光学效应极小。
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Statistical effects in the absorption and optical activity of particulate suspensions.颗粒悬浮液吸收和旋光性中的统计效应。
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Differential absorption flattening optical effects are significant in the circular dichroism spectra of large membrane fragments.差分吸收平坦化光学效应在大膜片段的圆二色光谱中很显著。
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Analyses of circular dichroism spectra of membrane proteins.膜蛋白的圆二色光谱分析。
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Biochem J. 1993 Jan 1;289 ( Pt 1)(Pt 1):215-9. doi: 10.1042/bj2890215.

本文引用的文献

1
Further characterization of protein secondary structures in purple membrane by circular dichroism and polarized infrared spectroscopies.用圆二色性和偏振红外光谱进一步研究紫色膜中蛋白质二级结构的特征。
Biophys J. 1985 Dec;48(6):873-6. doi: 10.1016/S0006-3495(85)83848-0.
2
The flattening of the absorption spectrum of suspensions, as compared to that of solutions.与溶液相比，悬浮液吸收光谱的扁平化。
Biochim Biophys Acta. 1956 Jan;19(1):1-12. doi: 10.1016/0006-3002(56)90380-8.
3
Circular dichroism analyses of membrane proteins: an examination of differential light scattering and absorption flattening effects in large membrane vesicles and membrane sheets.膜蛋白的圆二色性分析：对大膜泡和膜片层中差分光散射和吸收平坦化效应的研究。
Anal Biochem. 1984 Nov 1;142(2):317-28. doi: 10.1016/0003-2697(84)90471-8.
4
Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilamellar vesicles.在小单层囊泡的圆二色光谱中，差分光散射和吸收平坦化光学效应极小。
Biochemistry. 1984 Jun 5;23(12):2667-73. doi: 10.1021/bi00307a020.
5
Artifacts in the measured optic activity of membrane suspensions.膜悬浮液测量旋光性中的假象。
Arch Biochem Biophys. 1971 Feb;142(2):481-8. doi: 10.1016/0003-9861(71)90511-x.
6
Protein conformation in biomembranes: optical rotation and absorption of membrane suspensions.生物膜中的蛋白质构象：膜悬浮液的旋光性和吸收特性
Biochim Biophys Acta. 1972 Feb 14;265(1):115-68. doi: 10.1016/0304-4157(72)90021-4.
7
An experimental method correcting for absorption flattening and scattering in suspensions of absorbing particles: circular dichroism and absorption spectra of hemoglobin in situ in red blood cells.一种校正吸收性颗粒悬浮液中吸收平坦化和散射的实验方法：红细胞内血红蛋白原位的圆二色性和吸收光谱
Biochemistry. 1976 Sep 21;15(19):4158-62. doi: 10.1021/bi00664a004.