School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, China.
Molecules. 2022 Jun 23;27(13):4031. doi: 10.3390/molecules27134031.
The deactivation of degrading and pectinolytic enzymes is crucial in the fruit juice industry. In commercial fruit juice production, a variety of approaches are applied to inactivate degradative enzymes. One of the most extensively utilized traditional procedures for improving the general acceptability of juice is thermal heat treatment. The utilization of a non-thermal pulsed electric field (PEF) as a promising technology for retaining the fresh-like qualities of juice by efficiently inactivating enzymes and bacteria will be discussed in this review. Induced structural alteration provides for energy savings, reduced raw material waste, and the development of new products. PEF alters the α-helix conformation and changes the active site of enzymes. Furthermore, PEF-treated juices restore enzymatic activity during storage due to either partial enzyme inactivation or the presence of PEF-resistant isozymes. The increase in activity sites caused by structural changes causes the enzymes to be hyperactivated. PEF pretreatments or their combination with other nonthermal techniques improve enzyme activation. For endogenous enzyme inactivation, a clean-label hurdle technology based on PEF and mild temperature could be utilized instead of harsh heat treatments. Furthermore, by substituting or combining conventional pasteurization with PEF technology for improved preservation of both fruit and vegetable juices, PEF technology has enormous economic potential. PEF treatment has advantages not only in terms of product quality but also in terms of manufacturing. Extending the shelf life simplifies production planning and broadens the product range significantly. Supermarkets can be served from the warehouse by increasing storage stability. As storage stability improves, set-up and cleaning durations decrease, and flexibility increases, with only minor product adjustments required throughout the manufacturing process.
降解酶和果胶酶的失活在果汁行业至关重要。在商业果汁生产中,采用了多种方法来使降解酶失活。提高果汁整体可接受性的最广泛应用的传统方法之一是热热处理。本文将讨论利用非热脉冲电场(PEF)作为一种有前途的技术,通过有效灭活酶和细菌来保留果汁的新鲜品质。诱导的结构改变可节省能源、减少原材料浪费并开发新产品。PEF 改变α-螺旋构象并改变酶的活性部位。此外,由于部分酶失活或存在 PEF 抗性同工酶,PEF 处理的果汁在储存过程中会恢复酶活性。结构变化引起的活性部位增加会导致酶超激活。PEF 预处理或与其他非热技术结合可改善酶的激活。对于内源性酶失活,可以使用基于 PEF 和温和温度的清洁标签障碍技术代替苛刻的热处理。此外,通过替代或结合常规巴氏杀菌与 PEF 技术,可显著改善水果和蔬菜汁的保存效果,PEF 技术具有巨大的经济潜力。PEF 处理不仅在产品质量方面具有优势,而且在制造方面也具有优势。延长保质期可简化生产计划并显著扩大产品范围。通过提高存储稳定性,可以从仓库向超市供应产品。随着存储稳定性的提高,设置和清洁时间减少,灵活性提高,整个制造过程只需进行微小的产品调整。