Basic Medical Research Laboratory, Kunming Medical University, the 920th Hospital of Joint Logistics Support Force, PLA, Kunming, Yunnan Province, China.
The Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions, the 920th Hospital of Joint Logistics Support Force, PLA, Kunming, Yunnan Province, China.
Cell Mol Biol (Noisy-le-grand). 2022 Jan 2;67(4):358-366. doi: 10.14715/cmb/2021.67.5.41.
The aim of this study was to objective to investigate the effects of umbilical cord mesenchymal stem cells on the expression of CYR61, FSH and AMH in mice with premature ovarian failure. For this purpose, thirty SPF female SD mice were selected as the research object, 10 of which were control group, namely group α, and 20 mice with premature ovarian failure model were established by cyclophosphamide. The mice were divided into model group, namely β group and the umbilical cord mesenchymal stem cell transplantation group (γ group), with 10 mice in each group. ELSA method was used to determine the levels of follicle-stimulating hormone (FSH), Luteinizing hormone (LH), estradiol (Estradiol) in serum. The changes of E2, Antimullerian hormone (AMH) and cysteine-rich protein 61 in ovarian tissues were determined by the protein imprinting method. Connective tissue growth factor (CTGF) and caspase-3 protein expression. Results showed that in fertility rate, γ group > α group > β group, the difference was statistically significant (P<0.05), in litter size, α group > γ group > β group, the difference was statistically significant (P<0.05). The levels of serum E2 and AMH in α group > γ group > β group, and the levels of serum FSH and LH in β group > γ group > α group were statistically significant (P<0.05). The growth follicles were α group > γ group > β group, and the atresia follicles were β group > γ group > α group, and there was a statistical difference among all groups (P<0.05). There was no difference in luteal number among the three groups (P>0.05). In terms of CYR61 and CTGF protein expression, α group > γ group > β group, and in terms of caspase-3, β group > γ group > α group had statistical significance (P<0.05). It is concluded that intervention with umbilical cord mesenchymal stem cells can significantly improve the expression levels of CYR61 and AMH, reduce the level of FSH, promote cell survival, improve the reproductive quality of mice, and restore the physiological function of the ovary. It is feasible to treat premature ovarian failure with umbilical cord mesenchymal stem cells.
本研究旨在探讨脐带间充质干细胞对卵巢早衰小鼠 CYR61、FSH 和 AMH 表达的影响。为此，选择 30 只 SPF 雌性 SD 小鼠作为研究对象，其中 10 只为对照组，即α组，20 只通过环磷酰胺建立卵巢早衰模型鼠。将模型鼠分为模型组，即β组和脐带间充质干细胞移植组（γ组），每组 10 只。采用 ELSA 法测定血清中卵泡刺激素（FSH）、黄体生成素（LH）、雌二醇（Estradiol）水平。采用蛋白印迹法测定卵巢组织中 E2、抗苗勒管激素（AMH）和富含半胱氨酸蛋白 61 的变化。测定结缔组织生长因子（CTGF）和半胱天冬酶-3 蛋白表达。结果表明，在受孕率方面，γ组>α组>β组，差异有统计学意义（P<0.05），在产仔数方面，α组>γ组>β组，差异有统计学意义（P<0.05）。α组血清 E2 和 AMH 水平>γ组>β组，β组血清 FSH 和 LH 水平>γ组>α组，差异均有统计学意义（P<0.05）。生长卵泡为α组>γ组>β组，闭锁卵泡为β组>γ组>α组，各组间差异有统计学意义（P<0.05）。三组黄体数差异无统计学意义（P>0.05）。在 CYR61 和 CTGF 蛋白表达方面，α组>γ组>β组，在半胱天冬酶-3 方面，β组>γ组>α组，差异均有统计学意义（P<0.05）。结论：脐带间充质干细胞干预可显著提高 CYR61 和 AMH 的表达水平，降低 FSH 水平，促进细胞存活，提高小鼠生殖质量，恢复卵巢生理功能，用脐带间充质干细胞治疗卵巢早衰是可行的。
