Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China.
Department of Orthopedics, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Nanjing, 210014, China.
Cell Mol Biol (Noisy-le-grand). 2022 Jan 2;67(4):135-142. doi: 10.14715/cmb/2021.67.4.16.
Spinal tuberculosis or tuberculous spondylitis is one of the most common types of skeletal tuberculosis. Complications of the spine and spinal cord tuberculosis include destruction of the vertebrae, deformity, and paraplegia. Since in some patients, the clinical manifestations of tuberculosis are unusual and timely diagnosis and treatment of this disease can prevent its serious consequences, so in the present study, some cases of rare manifestations of tuberculosis were investigated. The expression of the NF-κB gene in these patients was also evaluated. In this regard, 36 patients with spinal tuberculosis and 30 healthy individuals (as a control group) were assessed. Clinical symptoms, imaging, laboratory tests, pathology, and response to treatment related to patients with spinal tuberculosis and spinal cord tuberculosis were evaluated. NF-κB expression was also evaluated using the PCR technique in peripheral white blood cell samples. The obtained results were analyzed using SPSS ver. 16, χ2 and T-test statistical methods. Mann-Whitney U test and Kruskal-Wallis non-parametric tests were used to analyze non-parametric data. The results showed that out of 36 cases of spinal tuberculosis, 29 cases had spinal tuberculosis, five cases had tuberculous radiculomyelitis, one case had spinal intramedullary tuberculoma, and one case had syringomyelia. 52.78% of patients were male, and 70% of cases were observed between the ages of 35 and 55 years. Fever and back pain were seen in more than 80% of cases. The study of NF-κB expression in the control and case groups showed that the NF-κB expression in the case group increased compared to the control group. This increase was statistically significant (P = 0.0071). In general, in the present study, the methods of clinical diagnosis of spinal tuberculosis were evaluated. Also, the amount of NF-κB transcription factor was evaluated as an effective genetic factor in the diagnosis of this disease.
脊柱结核或结核性脊柱炎是最常见的骨结核类型之一。脊柱和脊髓结核的并发症包括椎体破坏、畸形和截瘫。由于在某些患者中，结核病的临床表现不典型，及时诊断和治疗这种疾病可以预防其严重后果，因此在本研究中，调查了一些罕见表现形式的结核病病例。还评估了这些患者的 NF-κB 基因表达。为此，评估了 36 例脊柱结核患者和 30 名健康个体（作为对照组）。评估了与脊柱结核和脊髓结核患者相关的临床症状、影像学、实验室检查、病理学和治疗反应。还使用聚合酶链反应（PCR）技术评估了外周白细胞样本中的 NF-κB 表达。使用 SPSS ver.16 分析了获得的结果，使用 χ2 和 T 检验统计方法。使用 Mann-Whitney U 检验和 Kruskal-Wallis 非参数检验分析非参数数据。结果显示，在 36 例脊柱结核中，29 例为脊柱结核，5 例为结核性神经根脊髓炎，1 例为脊髓内结核瘤，1 例为脊髓空洞症。52.78%的患者为男性，70%的病例发生在 35 至 55 岁之间。超过 80%的病例出现发热和背痛。在对照组和病例组中研究 NF-κB 表达显示，病例组的 NF-κB 表达与对照组相比增加。这种增加具有统计学意义（P=0.0071）。总的来说，在本研究中，评估了脊柱结核的临床诊断方法。此外，还评估了 NF-κB 转录因子的数量作为诊断这种疾病的有效遗传因素。
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