Department of Biochemistry, College of Medicine, University of Hail, Hail, Kingdom of Saudi Arabia.
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.
Cell Mol Biol (Noisy-le-grand). 2022 Jan 2;67(4):42-55. doi: 10.14715/cmb/2021.67.4.6.
In view of many complications of diabetes, kidney failure is considered as one of the main complications. The oxidative stress-induced due to persistent hyperglycemic conditions is the major cause of kidney disease. The present study was designed to explore the nephroprotective efficacy of polyherbal (PH) extract in a diabetic model induced by streptozotocin (STZ). STZ (55 mg/kg body weight, intraperitoneal) was injected in overnight fasting rats to develop the diabetic experimental model. Effect on kidney injury was evaluated by investigating biochemical and histological evidences in renal tissue after 56 days of treatment of PH extract. Results showed the high glucose level in STZ treated rats that suggested hyperglycemia persistence along with the successful establishment of nephropathy in diabetic rats with altered renal function, inflammatory cytokines level as well as oxidative and nitrosative stress. Administration of PH extract significantly improved the glycemic condition, glomerular function and proximal reabsorptive markers. Further, elevated pro-inflammatory cytokines levels and disturbed redox status were restored. Moreover, findings were fostered and substantiated by histopathological examinations. Our work strongly proposes that the nephroprotective effect of the PH extract on renal damage could be attributed due to its anti-inflammatory and antioxidant properties. Thus, PH extract could have potential as a pharmaceutical drug for diabetes mellitus (DM). Additional long-term study or clinical trial is required for further investigations.
鉴于糖尿病的许多并发症，肾衰竭被认为是主要并发症之一。持续高血糖引起的氧化应激是导致肾脏疾病的主要原因。本研究旨在探讨多糖（PH）提取物在链脲佐菌素（STZ）诱导的糖尿病模型中的肾脏保护作用。STZ（55mg/kg 体重，腹腔注射）注射到禁食过夜的大鼠中，以建立糖尿病实验模型。通过研究 PH 提取物治疗 56 天后肾组织的生化和组织学证据，评估其对肾脏损伤的影响。结果表明，STZ 处理的大鼠血糖水平升高，提示高血糖持续存在，同时糖尿病大鼠的肾功能、炎症细胞因子水平以及氧化和硝化应激发生了改变，导致了肾病的发生。PH 提取物的给药显著改善了血糖状况、肾小球功能和近端重吸收标志物。此外，升高的促炎细胞因子水平和紊乱的氧化还原状态得到了恢复。此外，组织病理学检查结果也支持了这一发现。我们的工作有力地表明，PH 提取物对肾脏损伤的肾脏保护作用可能归因于其抗炎和抗氧化特性。因此，PH 提取物可能有作为治疗糖尿病（DM）的药物的潜力。需要进行进一步的长期研究或临床试验。
