Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, 13125, Berlin, Germany.
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, 13125, Berlin, Germany; German Center for Cardiovascular Research (DZHK), Partner Site Berlin, Germany; Charité University Medicine, Charitéplatz 1, 10117, Berlin, Germany; University of Lübeck, Institute for Biology, Ratzeburger Allee 160, 23538, Lübeck, Germany.
Biochem Biophys Res Commun. 2022 Sep 17;621:20-24. doi: 10.1016/j.bbrc.2022.06.082. Epub 2022 Jul 2.
Atherosclerosis has become prevalent not only in Western industrialized countries but all over the globe. Gpnmb, a transmembrane protein expressed by macrophages, has been detected in aortic lesions. We created an ApoE/Gpnmb-double knockout mouse using Crispr-Cas9 to examine the effect of Gpnmb deficiency on the development of atherosclerotic plaques. Feeding female mice a high cholesterol diet for 8 and 12 weeks, we detected an increased plaque size in aortic root sections of Gpnmb-deficient compared to control mice. However, the plaque area in whole thoracic and abdominal aorta was not different. Despite its strong expression in macrophages in aortic plaques, Gpnmb exerts only a minor effect on the growth of the atherosclerotic plaques in female mice. Future studies should examine plaque stability and include both sexes to elucidate the sex-specific function of Gpnmb in atherosclerosis.
动脉粥样硬化不仅在西方工业化国家普遍存在，在全球范围内也普遍存在。Gpnmb 是一种在巨噬细胞中表达的跨膜蛋白，已在主动脉病变中被检测到。我们使用 Crispr-Cas9 技术创建了 ApoE/Gpnmb 双敲除小鼠，以研究 Gpnmb 缺乏对动脉粥样硬化斑块发展的影响。用高胆固醇饮食喂养雌性小鼠 8 周和 12 周后，我们发现与对照组相比，Gpnmb 缺陷小鼠主动脉根部斑块的大小增加。然而，整个胸主动脉和腹主动脉的斑块面积没有差异。尽管 Gpnmb 在主动脉斑块中的巨噬细胞中表达强烈，但它对雌性小鼠动脉粥样硬化斑块的生长仅产生较小的影响。未来的研究应该检查斑块的稳定性，并包括两性，以阐明 Gpnmb 在动脉粥样硬化中的性别特异性功能。
