Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, PR China; Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, PR China.
J Environ Manage. 2022 Oct 1;319:115640. doi: 10.1016/j.jenvman.2022.115640. Epub 2022 Jul 6.
Black soils (Mollisols) are among the most important soil resources for crop production and food security. In China, they are mainly distributed in the northeastern region. To investigate soil antibiotic resistance distribution patterns and monitor soil quality, we randomly chose nine corn fields in Northeast China and analyzed the antibiotic resistance gene (ARG) distribution and co-occurrence patterns on the basis of high-throughput approaches and network analyses. High genetic diversity (136 unique genes) and low ARG abundances (10-10 copies/16S rRNA gene copy) were detected, with relatively few interactions among ARGs. Type I integron genes were prevalent in the soil and were positively correlated with ARGs, which may increase the risk of ARG transmission. Most ARGs were strongly associated with microorganisms. Moreover, several ARGs were significantly correlated with antibiotics, nutrients, and metal elements. The generation and dissemination of ARGs, which were most likely mediated by mobile genetic elements (MGEs) and bacteria, were affected by environmental conditions. These results provide insights into the widespread co-occurrence patterns in soil resistomes.
黑土（软土）是作物生产和粮食安全最重要的土壤资源之一。在中国，它们主要分布在东北地区。为了调查土壤抗生素抗性分布模式和监测土壤质量，我们随机选择了中国东北的九个玉米田，基于高通量方法和网络分析，分析了抗生素抗性基因（ARG）的分布和共存模式。检测到高遗传多样性（136 个独特基因）和低 ARG 丰度（10-10 拷贝/16S rRNA 基因拷贝），ARG 之间的相互作用相对较少。土壤中普遍存在 I 型整合子基因，与 ARGs 呈正相关，这可能增加 ARG 传播的风险。大多数 ARGs 与微生物密切相关。此外，一些 ARGs 与抗生素、营养物质和金属元素显著相关。ARG 的产生和传播最有可能是由移动遗传元件（MGE）和细菌介导的，受环境条件的影响。这些结果为土壤抗药性组中的广泛共存模式提供了深入的了解。
