Moody-Corbett F, Brehm P
Can J Physiol Pharmacol. 1987 Mar;65(3):348-51. doi: 10.1139/y87-060.
Cultures prepared from dissociated rat thymus were examined 1-2 weeks after plating. Macrophage cells were identified by their adherence, morphological appearance, and ability to phagocytize carbon particles or heat-inactivated Staphylococcus aureus. Whole cell current recordings from macrophage cells revealed an inward current at potentials more negative than the equilibrium potential for potassium and an outward current at potentials more positive than -40 mV in normal recording solution. Acetylcholine or muscarine caused a reduction in inward current but did not alter the outward current. The inward current and acetylcholine effect were seen at less negative potentials by decreasing the potassium equilibrium potential and both were blocked by the addition of cesium to the external recording solution. These results indicated that the inward current was mediated by potassium through the inward or anomalous rectifier. Physiologically, the action of acetylcholine on the inward rectifier of these macrophage cells may be mediated by cholinergic innervation of the thymus.
对从解离的大鼠胸腺制备的培养物在接种后1 - 2周进行检查。巨噬细胞通过其黏附性、形态外观以及吞噬碳颗粒或热灭活金黄色葡萄球菌的能力来鉴定。巨噬细胞的全细胞电流记录显示，在正常记录溶液中，当电位比钾的平衡电位更负时出现内向电流，当电位比 - 40 mV更正时出现外向电流。乙酰胆碱或毒蕈碱会导致内向电流减小，但不改变外向电流。通过降低钾平衡电位，在较不负极性的电位下可观察到内向电流和乙酰胆碱效应，并且在外部记录溶液中添加铯可阻断这两者。这些结果表明，内向电流是由钾通过内向或异常整流器介导的。从生理角度来看，乙酰胆碱对这些巨噬细胞内向整流器的作用可能是由胸腺的胆碱能神经支配介导的。
