Wu Jinhai, Zhao Jiamei, Liu Mingzhu, Zhao Zunquan, Qiu Yu, Li Hanle, Wu Jin, Bai Jialei
College of Food Science Sciences, Shanxi Normal University, Taiyuan City, China.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, China.
Biosci Biotechnol Biochem. 2022 Aug 24;86(9):1192-1199. doi: 10.1093/bbb/zbac112.
We developed a new ochratoxin A (OTA) aptamer biosensor to promptly detect OTA in food. Mesoporous silica nanoparticles were used as carriers, and aptamers were used as recognition probes and gating molecules. The fluorescent dye rhodamine 6G was loaded into mesoporous silica, and through electrostatic contact, the OTA aptamer was adsorbed on amino-modified mesoporous silica. The fluorescent dye released from the mesopore in the presence of OTA because of the conformational change induced in the aptamer by the target. The amount of ochratoxin was determined by measuring the fluorescence intensity. Our findings revealed a positive relationship between the fluorescence intensity and OTA concentration, with a limit of detection of 0.28 ng mL-1, and the detection range was 0.05-200 ng mL-1. The recovery rate was 80.7%-110.8% in real samples. The proposed approach is suitable for the quantification of other toxins.
我们开发了一种新型赭曲霉毒素A（OTA）适配体生物传感器，用于快速检测食品中的OTA。以介孔二氧化硅纳米颗粒为载体，以适配体作为识别探针和门控分子。将荧光染料罗丹明6G负载到介孔二氧化硅中，通过静电作用，OTA适配体吸附在氨基修饰的介孔二氧化硅上。在目标物OTA存在的情况下，由于适配体构象发生变化，介孔中的荧光染料释放出来。通过测量荧光强度来测定赭曲霉毒素的含量。我们的研究结果表明，荧光强度与OTA浓度呈正相关，检测限为0.28 ng mL-1，检测范围为0.05 - 200 ng mL-1。实际样品中的回收率为80.7% - 110.8%。所提出的方法适用于其他毒素的定量分析。
