Seyfried T N, Yu R K, Saito M, Albert M
Cancer Res. 1987 Jul 1;47(13):3538-42.
The ganglioside composition of an experimental ependymoblastoma was examined in C57BL/6 mice. This tumor was produced by Dr. H. Zimmerman in 1949 from methylcholanthrene implantation in the brain and has been maintained in serial transplants through many generations. The influence of tumor environment on ganglioside composition was determined by studying the tumor growing intracerebrally and s.c. (over the skull and in the flank). The ganglioside composition of this tumor is markedly different from that of adult mouse brain. The total ganglioside sialic acid content (micrograms/100 mg dry weight) of the tumor growing in the cerebrum, s.c. over the skull, and in the flank was 70.4 +/- 3.8 (N = 3), 66.8 (N = 2), and 41.7 +/- 0.7 (N = 3), respectively. These values are about 10-fold lower than the ganglioside content of normal mouse cerebrum. This tumor contained a significant amount of N-glycolyneuraminic acid (NGNA). Histological analysis revealed two basically different cell types. The predominant cell type is densely packed and poorly defined in shape, whereas the minor cell type is less densely packed and fibroblastlike in shape. GM3, which migrates as double bands on thin-layer chromatography, is the predominant ganglioside of this tumor in all three regions of growth. Also present in all regions are gangliosides NGNA-GM3 and GM1. Significant amounts of GD1a, GD1b, GT1b, and GQ1b are present only in the cerebral tumor. These gangliosides therefore represent contaminants from normal brain tissue surrounding the tumor and are not native to the tumor. Ganglioside GD3, however, is a minor component of the tumor. Using a thin-layer chromatography-immunostaining method with anti-GA1 antibody, we found significant amounts of ganglioside with a GA1 oligosaccharide backbone migrating near GD3 and GD2. This tumor is similar to other neural tumors in having elevated amounts of GM3 and reduced amounts of total ganglioside and polysialogangliosides but is unique in having a high content of NGNA-containing gangliosides. The possible origin of the NGNA-containing gangliosides is discussed.
在C57BL/6小鼠中检测了实验性室管膜母细胞瘤的神经节苷脂组成。该肿瘤由H. Zimmerman博士于1949年通过将甲基胆蒽植入脑内产生，并通过多代连续移植得以维持。通过研究在脑内和皮下（颅骨上方和侧腹）生长的肿瘤，确定了肿瘤环境对神经节苷脂组成的影响。该肿瘤的神经节苷脂组成与成年小鼠脑的神经节苷脂组成明显不同。在大脑中、颅骨上方皮下以及侧腹生长的肿瘤的总神经节苷脂唾液酸含量（微克/100毫克干重）分别为70.4±3.8（N = 3）、66.8（N = 2）和41.7±0.7（N = 3）。这些值比正常小鼠大脑的神经节苷脂含量低约10倍。该肿瘤含有大量的N - 羟乙酰神经氨酸（NGNA）。组织学分析揭示了两种基本不同的细胞类型。主要细胞类型紧密堆积且形状难以界定，而次要细胞类型堆积较松散且呈成纤维细胞样形状。在薄层色谱上以双带形式迁移的GM3是该肿瘤在所有三个生长区域中的主要神经节苷脂。所有区域中还存在神经节苷脂NGNA - GM3和GM1。仅在脑肿瘤中存在大量的GD1a、GD1b、GT1b和GQ1b。因此，这些神经节苷脂代表来自肿瘤周围正常脑组织的污染物，而非肿瘤本身所固有。然而，神经节苷脂GD3是肿瘤的次要成分。使用抗GA1抗体的薄层色谱 - 免疫染色方法，我们发现大量具有GA1寡糖主链的神经节苷脂在GD3和GD2附近迁移。该肿瘤与其他神经肿瘤相似，具有升高的GM3含量以及降低的总神经节苷脂和多唾液酸神经节苷脂含量，但独特之处在于含有高含量的含NGNA神经节苷脂。文中讨论了含NGNA神经节苷脂的可能来源。
