Chen Long, Liu AnFeng, Guo ZiWen, Jiang Hui, Luo Ling, Gao JunShan, Li DaHui, Ye SiHong, Guo Ning
School of Life Sciences, Anhui Agricultural University, Hefei, China.
Cotton Research Institute of Anhui Academy of Agricultural Sciences, Hefei, China.
Front Plant Sci. 2022 Jun 24;13:841161. doi: 10.3389/fpls.2022.841161. eCollection 2022.
With the continuous growth of the human population, the demand for fiber is also rising sharply. As one of the main fiber plants available globally, cotton fiber yield () is affected by boll abscission, which is related to the formation of the abscission layer. Therefore, we explored the formation of the abscission layer in cotton. The formation of the abscission layer in the cotton boll stalk was promoted by exogenous ethylene. It was found that both the number of the Golgi apparatus and the number of stacking layers increased in the dissociated cells. The gene family in cotton was screened by the bioinformatics method, and the species and evolutionary relationship of gene family were analyzed. qRT-PCR showed that , , , and in cotton had spatiotemporal-specific expression patterns. Subcellular localization suggested that played a role in the Golgi apparatus. The expression of in transgenic is increased in the roots, stems, and leaves. Finally, we found that ethylene could induce the formation of the abscission layer in cotton. , and might regulate the changes in the Golgi apparatus in the abscission layer. Taken together, the findings provide new ideas for the study of the formation of cotton abscission.
随着人口的持续增长,对纤维的需求也在急剧上升。作为全球主要的纤维植物之一,棉花纤维产量()受到落铃的影响,而落铃与离层的形成有关。因此,我们探究了棉花离层的形成。外源乙烯促进了棉铃柄中离层的形成。研究发现,解离细胞中高尔基体的数量和堆叠层数均增加。通过生物信息学方法筛选了棉花中的基因家族,并分析了基因家族的种类和进化关系。qRT-PCR表明,棉花中的、、和具有时空特异性表达模式。亚细胞定位表明在高尔基体中发挥作用。在转基因中的表达在根、茎和叶中均增加。最后,我们发现乙烯可诱导棉花离层的形成。、和可能调控离层中高尔基体的变化。综上所述,这些发现为棉花离层形成的研究提供了新思路。