Keough D T, McConachie L A, Gordon R B, de Jersey J, Emmerson B T
Clin Chim Acta. 1987 Mar 30;163(3):301-8. doi: 10.1016/0009-8981(87)90248-8.
A simple and rapid spectrophotometric assay for the estimation of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity in human tissues is described. It is based on the increase in absorbance at 257.5 nm which occurs when the substrate guanine is converted to its 5'-mononucleotide, GMP. The assay has been developed to measure HGPRT activity in erythrocyte and lymphocyte lysates and in brain homogenates, and has been used in the screening of patients with hyperuricaemia and/or hyperuricosuria for HGPRT deficiency. It has also been used to determine the steady-state kinetic constants of a mutant form of the enzyme. The spectrophotometric assay is compared with the radioactive assay currently used to measure HGPRT activity.
本文描述了一种用于估算人体组织中次黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶（HGPRT）活性的简单快速分光光度测定法。该方法基于当底物鸟嘌呤转化为其5'-单核苷酸GMP时，在257.5 nm处吸光度的增加。此测定法已用于测量红细胞和淋巴细胞裂解物以及脑匀浆中的HGPRT活性，并已用于筛查高尿酸血症和/或高尿酸尿症患者的HGPRT缺乏症。它还被用于测定该酶突变形式的稳态动力学常数。将该分光光度测定法与目前用于测量HGPRT活性的放射性测定法进行了比较。
