来自[具体来源1]和[具体来源2]的新型微小RNA及其功能靶点。
Novel MicroRNAs and their Functional Targets from and .
作者信息
Varghese Binta, Valsalan Ravisankar, Mathew Deepu
机构信息
Bioinformatics Centre, Kerala Agricultural University, Thrissur - 680 656, India.
出版信息
Curr Genomics. 2022 Apr 7;23(1):41-49. doi: 10.2174/1389202923666211223122305.
BACKGROUND
Even though miRNAs play vital roles in developmental biology by regulating the translation of mRNAs, they are poorly studied in oomycetes, especially in the plant pathogen .
OBJECTIVE
The study aimed to predict and identify the putative miRNAs and their targets in and .
METHODS
The homology-based comparative method was used to identify the unique miRNA sequences in and with 148,689 EST and TSA sequences of these species. Secondary structure prediction of sRNAs for the 76 resultant sequences has been performed with the MFOLD tool, and their targets were predicted using psRNATarget.
RESULTS
Novel miRNAs, miR-8210 and miR-4968, were predicted from and , respectively, along with their structural features. The newly identified miRNAs were identified to play important roles in gene regulation, with few of their target genes predicted as transcription factors, tumor suppressor genes, stress-responsive genes, DNA repair genes, .
CONCLUSION
The miRNAs and their targets identified have opened new interference and editing targets for the development of resistant crop varieties.
背景
尽管微小RNA（miRNAs）通过调节信使核糖核酸（mRNAs）的翻译在发育生物学中发挥着至关重要的作用，但它们在卵菌纲中研究较少，尤其是在植物病原体中。
目的
本研究旨在预测和鉴定[具体物种1]和[具体物种2]中的假定miRNAs及其靶标。
方法
采用基于同源性的比较方法，利用这两个物种的148,689条表达序列标签（EST）和转录组枪击装配（TSA）序列来鉴定[具体物种1]和[具体物种2]中独特的miRNA序列。使用MFOLD工具对76条所得序列的小RNA（sRNAs）进行二级结构预测，并使用psRNATarget预测其靶标。
结果
分别从[具体物种1]和[具体物种2]中预测出新型miRNAs，即miR - 8210和miR - 4968，以及它们的结构特征。新鉴定的miRNAs被确定在基因调控中发挥重要作用，其预测的靶基因中很少有转录因子、肿瘤抑制基因、应激反应基因、DNA修复基因等。
结论
所鉴定的miRNAs及其靶标为抗[具体物种]作物品种的开发开辟了新的干扰和编辑靶点。
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