探索古代植物的秘密：从博物馆和考古植物标本中回收 DNA。

Discovering the Secrets of Ancient Plants: Recovery of DNA from Museum and Archaeological Plant Specimens.

机构信息

Centre for Anthropobiology and Genomics of Toulouse (CAGT), CNRS UMR 5288, Université Toulouse III - Paul Sabatier, Toulouse, France.

Australian Centre for Ancient DNA (ACAD), School of Biological Science, The University of Adelaide, Adelaide, SA, Australia.

出版信息

Methods Mol Biol. 2022;2512:261-267. doi: 10.1007/978-1-0716-2429-6_15.

DOI:10.1007/978-1-0716-2429-6_15

PMID:35818010

Abstract

Plant DNA preserved in ancient specimens has recently gained importance as a tool in comparative genomics, allowing the investigation of evolutionary processes in plant genomes through time. However, recovering the genomic information contained in such specimens is challenging owing to the presence of secondary substances that limit DNA retrieval. In this chapter, we provide a DNA extraction protocol optimized for the recovery of DNA from degraded plant materials. The protocol is based on a commercially available DNA extraction kit that does not require handling of hazardous reagents.

摘要

近年来，保存在古代标本中的植物 DNA 作为比较基因组学的工具得到了重视，它可以通过研究植物基因组随时间的进化过程。然而，由于存在限制 DNA 提取的次生物质，从这些标本中获取基因组信息具有一定的挑战性。在本章中，我们提供了一种针对从降解植物材料中回收 DNA 进行了优化的 DNA 提取方案。该方案基于一种商业上可用的 DNA 提取试剂盒，它不需要处理危险试剂。