Department of Nursing, Tangshan Vocational and Technical College, Tangshan 063000, China.
EEG Room, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, China.
Cell Mol Biol (Noisy-le-grand). 2022 Feb 27;67(6):191-199. doi: 10.14715/cmb/2021.67.6.26.
This study aimed to investigate the effect of Solanum lyratum polysaccharide on the malignant behavior of lung cancer cells and its possible mechanism. For this purpose, lung cancer A549 cells were cultured in vitro and treated with different doses (0.4, 0.8, 1.2 mg/mL) of Solanum lyratum polysaccharide for 24 h. Then cell proliferation was detected by the CCK-8 method and clone formation test. Transwell test was used to detect cell migration and invasion, and flow cytometry was used to detect cell apoptosis. The protein expressions of Bax and Bcl-2 in cells were detected by Western blotting, and the protein expressions of circ_UHRF1 and miR-513b-5p were detected by the qRT-PCR method. Pearson correlation was used to analyze the correlation between circ_UHRF1 and miR-513b-5p expressions in lung cancer tissues. Results showed that compared with the control group, the proliferation inhibition rate and apoptosis rate of A549 cells that intervened with the Solanum lyratum polysaccharide and expression of Bax protein in the cells were all increased (P<0.05), but the number of clones, migration and invasion and the protein expression of Bcl-2 were all decreased (P<0.05), and were dose-dependent. The expression of circ_UHRF1 in A549 cells that intervened with the S. lyratum polysaccharide was decreased (P<0.05), but the expression of miR-513b-5p was increased (P<0.05). The expression of circ_UHRF1 in lung cancer tissues was higher than that of adjacent tissues (P<0.05), and the expression of miR-513b-5p was lower than that of adjacent tissues (P<0.05). The expressions of circ_UHRF1 and miR-513b-5p in lung cancer tissues were negatively correlated (r=-0.861, P<0.05). Circ_UHRF1 could target miR-513b-5p, and the expression of miR-513b-5p in A549 cells knocking down circ_UHRF1 was increased. After knocking down circ_UHRF1, the proliferation inhibition rate and apoptosis rate of A549 cells and protein expression of Bax in the cells were all increased (P<0.05), but the number of clones, migration and invasion and the protein expression of Bcl-2 were all decreased (P<0.05). Up-regulation of circ_UHRF1 reduced the effects of S. lyratum polysaccharide on the proliferation, migration, invasion and apoptosis of A549 cells. In general, S. lyratum polysaccharide could inhibit the proliferation, migration and invasion of lung cancer A549 cells, and induce cell apoptosis. Its mechanism may be related to the regulation of the circ_UHRF1/miR-513b-5p axis.
本研究旨在探讨龙葵多糖对肺癌细胞恶性行为的影响及其可能的机制。为此，将肺癌 A549 细胞在体外进行培养，并分别用不同剂量（0.4、0.8、1.2 mg/mL）的龙葵多糖处理 24 h。然后用 CCK-8 法和克隆形成实验检测细胞增殖，用 Transwell 实验检测细胞迁移和侵袭，用流式细胞术检测细胞凋亡。用 Western blot 检测细胞中 Bax 和 Bcl-2 蛋白的表达，用 qRT-PCR 法检测 circ_UHRF1 和 miR-513b-5p 的蛋白表达。采用 Pearson 相关分析肺癌组织中 circ_UHRF1 与 miR-513b-5p 表达的相关性。结果表明，与对照组相比，经龙葵多糖干预的 A549 细胞的增殖抑制率和细胞凋亡率均升高（P<0.05），但克隆数、迁移和侵袭以及 Bcl-2 蛋白的表达均降低（P<0.05），且呈剂量依赖性。经龙葵多糖干预的 A549 细胞中 circ_UHRF1 的表达降低（P<0.05），但 miR-513b-5p 的表达升高（P<0.05）。肺癌组织中 circ_UHRF1 的表达高于相邻组织（P<0.05），而 miR-513b-5p 的表达低于相邻组织（P<0.05）。肺癌组织中 circ_UHRF1 和 miR-513b-5p 的表达呈负相关（r=-0.861，P<0.05）。Circ_UHRF1 可以靶向 miR-513b-5p，敲低 circ_UHRF1 的 A549 细胞中 miR-513b-5p 的表达增加。敲低 circ_UHRF1 后，A549 细胞的增殖抑制率和细胞凋亡率以及细胞中 Bax 蛋白的表达均升高（P<0.05），但克隆数、迁移和侵袭以及 Bcl-2 蛋白的表达均降低（P<0.05）。Circ_UHRF1 的上调降低了龙葵多糖对 A549 细胞增殖、迁移、侵袭和凋亡的影响。综上所述，龙葵多糖可抑制肺癌 A549 细胞的增殖、迁移和侵袭，并诱导细胞凋亡。其机制可能与调节 circ_UHRF1/miR-513b-5p 轴有关。
