壳分析和线扫描测量溶酶体定位。
Measurement of Lysosome Positioning by Shell Analysis and Line Scan.
机构信息
Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Program in Cell Biology, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children, Toronto, ON, Canada.
出版信息
Methods Mol Biol. 2022;2473:285-306. doi: 10.1007/978-1-0716-2209-4_19.
Lysosomes are membrane-bound organelles that degrade diverse biomolecules and regulate a multitude of other essential processes including cell growth and metabolism, signaling, plasma membrane repair and infection. Such diverse functions of lysosomes are highly coordinated in space and time and are therefore tightly coupled to the directional transport of the organelles within the cytoplasm. Thus, robust quantitative assessments of lysosome positioning within the cell provide a valuable tool for researchers interested in understanding these multifunctional organelles. Here, we present point-by-point methodology to measure lysosome positioning by two straight forward and widely used techniques: shell analysis and line scan.
溶酶体是一种膜结合的细胞器，能够降解多种生物分子，并调节包括细胞生长和代谢、信号转导、质膜修复和感染在内的多种其他重要过程。溶酶体的这些多样化功能在空间和时间上高度协调，因此与细胞器在细胞质内的定向运输紧密耦合。因此，对细胞内溶酶体定位进行强有力的定量评估，为研究这些多功能细胞器的研究人员提供了一种有价值的工具。在这里，我们通过两种简单而广泛使用的技术——壳分析和线扫描，介绍了测量溶酶体定位的详细方法。
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