Phytochemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), PO CIMAP, Lucknow 226015, India; Academy of Scientific and Innovative Research, Ghaziabad 110025, India.
Crop Production and Protection Division, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), PO CIMAP, Lucknow 226015, India.
Food Chem. 2022 Dec 1;396:133647. doi: 10.1016/j.foodchem.2022.133647. Epub 2022 Jul 8.
Marigold (Tagetes erecta L.) petals are the primary industrial source of lutein, which is used as a colouring agent and nutrient supplement to foods. This research extracted marigold petals using different solvents, covering conventional and non-toxic green solvents. The oleoresin, free lutein, and recrystallized lutein yields varied from 8.47-16.67%, 2.56-9.62%, and 1.11-1.61%, respectively. The purity of lutein was achieved up to 92.57% and 97.64% in conventional and newly established green methods, respectively. The present study described an efficient green process to isolate lutein with significantly improved yield (2.56%) and purity (97.33%) over the conventional methods. Based on the results, 2-methyltetrahydrofurancould be a practical green alternative to the traditional toxic solvents for the processing of lutein. Further, the chemical analysis of the essential oil of the residual receptacles obtained after removing petals revealed the presence of important organic volatiles, including piperitone (54.7%) and piperitenone oxide (6.5%), indicating its usefulness for value-addition.
万寿菊花瓣是叶黄素的主要工业来源，叶黄素被用作食品的着色剂和营养补充剂。本研究使用不同的溶剂（包括常规溶剂和无毒绿色溶剂）提取万寿菊花瓣。所得的油树脂、游离叶黄素和重结晶叶黄素的产率分别为 8.47-16.67%、2.56-9.62%和 1.11-1.61%。叶黄素的纯度在常规方法和新建立的绿色方法中分别达到 92.57%和 97.64%。本研究描述了一种从万寿菊花瓣中分离叶黄素的有效绿色工艺，与传统方法相比，该工艺显著提高了叶黄素的产量（2.56%）和纯度（97.33%）。基于这些结果，2-甲基四氢呋喃可以作为传统有毒溶剂的实用绿色替代品，用于加工叶黄素。此外，从去除花瓣后获得的残余容器的精油的化学分析表明，存在重要的有机挥发物，包括胡椒酮（54.7%）和胡椒烯酮氧化物（6.5%），表明其具有附加值的用途。
