Zhang Q H, Zhao W P
Zhonghua Zhong Liu Za Zhi. 1986 Nov;8(6):424-6.
It is difficult to diagnose malignant chromaffinoma among the endocrine tumors. At present, using biochemical and histochemical methods, tumors with the estrogen or nonestrogen target organs have been proved to possess estradiol receptors. In this paper, binding of steroid to active chromaffinoma was observed using synthesized 4-amino-N-fluorescent isothiocyanate-17 beta-estradiol (4 NHEF) as a probe. Benign and malignant chromaffinoma may be differentiated by the fluorescence difference. Under the same condition, the binding of fluorescence estradiol to the normal adrenal cells was weak. It was stronger in the majority of benign chromaffinoma, but was very strong in the malignant type. In a case of malignant chromaffinoma, the adrenal was excised first which was diagnosed as a benign tumor by pathological examination. But it was malignant by 4 NHEF stain. After two days, the patient's blood pressure became elevated and a second operation had to be done. Still, the tumor cells were benign by pathological examination. This chromaffinoma was finally diagnosed by fluorescence labelled estradiol which is more sensitive than pathology.
在内分泌肿瘤中，恶性嗜铬细胞瘤很难诊断。目前，利用生化和组织化学方法已证实，具有雌激素或非雌激素靶器官的肿瘤含有雌二醇受体。本文以合成的4-氨基-N-异硫氰酸荧光素-17β-雌二醇（4-NHEF）为探针，观察了类固醇与活性嗜铬细胞瘤的结合情况。良性和恶性嗜铬细胞瘤可通过荧光差异来区分。在相同条件下，荧光雌二醇与正常肾上腺细胞的结合较弱。在大多数良性嗜铬细胞瘤中结合较强，但在恶性类型中结合非常强。有一例恶性嗜铬细胞瘤患者，首先切除肾上腺，经病理检查诊断为良性肿瘤。但经4-NHEF染色显示为恶性。两天后，患者血压升高，不得不进行二次手术。病理检查肿瘤细胞仍为良性。该嗜铬细胞瘤最终通过荧光标记雌二醇得以诊断，其比病理检查更敏感。
