Godinho R O, Lima-Landman M T, Souccar C, Lapa A J
Exp Neurol. 1987 Jun;96(3):558-68. doi: 10.1016/0014-4886(87)90218-4.
The effects of testosterone withdrawal and chronic denervation on muscle weight and acetylcholinesterase (AChE) activity were studied in the hormone-sensitive levator ani muscle of the rat. Castration of adult male rats for 7 to 60 days caused a linear decrease of the weight, protein content, and AChE activity of the muscle, which stabilized after 30 days. Muscle weight and protein content decreased 2.3% per day. The total AChE activity decreased 7 days later 3.2% per day, reaching 37% of control at day 30. AChE activity per unit weight was increased in all castrated groups. Muscle weights and AChE activity of the extensor digitorum longus and soleus muscles were not altered after castration. Denervation of all three muscles caused 50% reduction of the muscle weight and protein content after 15 days. Total AChE activity decayed exponentially with a rate of 0.12 per day to 15 to 18% of control values. AChE activity per unit weight in the denervated muscles was always lower than in the control muscles. Combined castration and denervation intensified only the levator ani protein loss. The different onset and time course of the effects induced by castration and denervation indicate distinct mechanisms involved in the trophic control of muscle proteins and AChE activity. Chronic muscle denervation decreased total AChE activity to 15% of normal, whereas castration reduced the enzyme to 40% of the control values. The results indicate that neuronal and hormonal influences on AChE activity of the levator ani are not additive but overlap.
在大鼠激素敏感的提肛肌中，研究了睾酮撤除和慢性去神经支配对肌肉重量和乙酰胆碱酯酶（AChE）活性的影响。成年雄性大鼠去势7至60天会导致该肌肉的重量、蛋白质含量和AChE活性呈线性下降，30天后趋于稳定。肌肉重量和蛋白质含量每天下降2.3%。总AChE活性在7天后每天下降3.2%，在第30天达到对照值的37%。所有去势组单位重量的AChE活性均升高。去势后，趾长伸肌和比目鱼肌的肌肉重量和AChE活性未发生改变。对所有三块肌肉进行去神经支配15天后，肌肉重量和蛋白质含量减少了50%。总AChE活性呈指数衰减，每天衰减率为0.12，降至对照值的15%至18%。去神经支配肌肉中单位重量的AChE活性始终低于对照肌肉。联合去势和去神经支配仅加剧了提肛肌蛋白质的损失。去势和去神经支配所诱导效应的不同起始时间和时间进程表明，在肌肉蛋白质和AChE活性的营养控制中涉及不同的机制。慢性肌肉去神经支配使总AChE活性降至正常水平的15%，而去势则将该酶活性降至对照值的40%。结果表明，神经元和激素对提肛肌AChE活性的影响并非相加而是重叠的。
