Rosenthal G A, Berge M A, Bleiler J A, Rudd T P
Experientia. 1987 May 15;43(5):558-61. doi: 10.1007/BF02143585.
L-Canavanine, 2-amino-4-(guanidinooxy)butyric acid, and L-arginine incorporation into de novo synthesized proteins was compared in six organisms. Utilizing L-[guanidinooxy14C]canavanine and L-[guanidino14C]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determined in de novo synthesized proteins. Caryedes brasiliensis and Sternechus tuberculatus, canavanine utilizing insects; Canavalia ensiformis, a canavanine storing plant; and to a lesser extent Heliothis virescens, a canavanine resistant insect, failed to accumulate significant canavanyl proteins. By contrast, Manduca sexta, a canavanine-sensitive insect, and Glycine max, a canavanine free plant, readily incorporated canavanine into newly synthesized proteins. This study supports the contention that the incorporation of canavanine into proteins in place of arginine contributes significantly to canavanine's antimetabolic properties.
在六种生物体中比较了L-刀豆氨酸、2-氨基-4-（胍基氧基）丁酸和L-精氨酸掺入从头合成蛋白质的情况。在底物饱和条件下使用L-[胍基氧基¹⁴C]刀豆氨酸和L-[胍基¹⁴C]精氨酸，测定了从头合成蛋白质中刀豆氨酸与精氨酸的掺入率。巴西豆象和瘤胸豆象，利用刀豆氨酸的昆虫；刀豆，一种储存刀豆氨酸的植物；以及在较小程度上，对刀豆氨酸有抗性的昆虫烟青虫，均未积累大量刀豆氨酰蛋白质。相比之下，对刀豆氨酸敏感的昆虫烟草天蛾和不含刀豆氨酸的植物大豆，很容易将刀豆氨酸掺入新合成的蛋白质中。这项研究支持了这样的观点，即刀豆氨酸取代精氨酸掺入蛋白质中对刀豆氨酸的抗代谢特性有显著贡献。
