Kärki T, Hakkola E, Hassinen I E, Hiltunen J K
FEBS Lett. 1987 May 11;215(2):228-32. doi: 10.1016/0014-5793(87)80151-5.
The metabolism of the double bonds at the delta 3 position in fatty acids was studied in rat liver. Infusion of delta 3-trans-dodecenoic acid into isolated perfused liver and subcellular fractionation studies showed the presence of both peroxisomal and mitochondrial delta 3,delta 2-enoyl-CoA isomerase activity (EC 5.3.3.8). These findings together with the previous demonstration of peroxisomal 2,4-dienoyl-CoA reductase (EC 1.3.1.34) [(1981) J. Biol. Chem. 256, 8259-8262] and D-3-OH-acyl-CoA epimerase (EC 5.1.2.3) [(1985) FEBS Lett. 185, 129-134] activities show that peroxisomes possess all the auxiliary enzymes required for the beta-oxidation of unsaturated fatty acids.
在大鼠肝脏中研究了脂肪酸δ3位双键的代谢。将δ3-反式-十二碳烯酸注入离体灌注肝脏并进行亚细胞分级分离研究，结果显示过氧化物酶体和线粒体中均存在δ3,δ2-烯酰辅酶A异构酶活性（EC 5.3.3.8）。这些发现与先前证明的过氧化物酶体2,4-二烯酰辅酶A还原酶（EC 1.3.1.34）[（1981年）《生物化学杂志》256, 8259 - 8262]和D-3-羟基酰基辅酶A差向异构酶（EC 5.1.2.3）[（1985年）《欧洲生物化学学会联合会快报》185, 129 - 134]的活性一起表明，过氧化物酶体拥有不饱和脂肪酸β氧化所需的所有辅助酶。
