Kothary R, Perry M D, Moran L A, Rossant J
Dev Biol. 1987 Jun;121(2):342-8. doi: 10.1016/0012-1606(87)90170-9.
Transcription of the mouse hsp68 and hsc70 genes in embryonal carcinoma cells, various embryonic and extraembryonic tissues, and some adult tissues has been assessed using cloned probes to the mouse hsp68 gene. The results from Northern blots showed that both F9 and P19 cells respond in the expected manner to a heat shock. Hsp68 expression was only detected in heat-induced F9 and P19 cells. Hsc70 transcripts were present in uninduced cells and their levels increased after induction. In adult tissues, the hsp68 gene was expressed constitutively in the kidney. A different hsp68-like transcript was detected at significant levels in adult testes. Constitutive expression of hsp68 was observed in both the placenta (beginning at Day 8.5) and yolk sac (beginning at Day 11.5). No hsp68 expression was detected in embryonic tissues until Day 15.5. Expression of the mouse hsp68 gene during embryogenesis suggests that it may play some role in development.
利用小鼠hsp68基因的克隆探针，对胚胎癌细胞、各种胚胎和胚外组织以及一些成体组织中该基因的转录情况进行了评估。Northern印迹法的结果表明，F9和P19细胞对热休克的反应符合预期。hsp68的表达仅在热诱导的F9和P19细胞中检测到。Hsc70转录本存在于未诱导的细胞中，诱导后其水平升高。在成体组织中，hsp68基因在肾脏中持续表达。在成年睾丸中检测到显著水平的一种不同的hsp68样转录本。在胎盘（从第8.5天开始）和卵黄囊（从第11.5天开始）中均观察到hsp68的组成型表达。直到第15.5天，胚胎组织中才检测到hsp68的表达。小鼠hsp68基因在胚胎发育过程中的表达表明它可能在发育中发挥某种作用。
