Bradford C S, Taylor M H
Gen Comp Endocrinol. 1987 Apr;66(1):71-8. doi: 10.1016/0016-6480(87)90351-0.
Radioimmunoassay techniques were used to measure estradiol-17 beta (E2) and cortisol in the serum and ovarian fluid of Fundulus heteroclitus throughout complete semilunar spawning cycles. The cyclic nature of spawning activity was confirmed by monitoring the gonadosomatic index and noting the times at which ovulated eggs were found within the ovarian lumen. Levels of serum E2, ovarian fluid E2, and serum cortisol all exhibited cycles which paralleled the semilunar cycle of gonadal maturity and spawning in this euryhaline killifish. Elevations of serum cortisol were temporally associated with ovulation, whereas ovarian fluid E2 levels peaked several days prior to ovulation. Serum E2 remained elevated after ovarian fluid E2 levels had returned to baseline. Cortisol concentrations in ovarian fluid did not cycle and no correlation was apparent between serum cortisol and glucose concentrations.
在整个半月形产卵周期中，采用放射免疫分析技术测定了底鳉血清和卵巢液中的17β-雌二醇（E2）和皮质醇。通过监测性腺体指数并记录在卵巢腔内发现排卵卵的时间，证实了产卵活动的周期性。血清E2、卵巢液E2和血清皮质醇水平均呈现出与这种广盐性鳉鱼性腺成熟和产卵的半月周期平行的周期。血清皮质醇的升高在时间上与排卵相关，而卵巢液E2水平在排卵前几天达到峰值。在卵巢液E2水平恢复到基线后，血清E2仍保持升高。卵巢液中的皮质醇浓度没有周期性变化，血清皮质醇与葡萄糖浓度之间也没有明显的相关性。
