Tsang P C, Callard I P
Gen Comp Endocrinol. 1987 May;66(2):182-9. doi: 10.1016/0016-6480(87)90266-8.
Pregnant female spiny dogfish were captured off the coasts of Maine and Massachusetts and morphometric and gravimetric analyses of the liver, gonad, and reproductive tract correlated with the reproductive cycle. Plasma samples were taken and circulating steroids (progesterone, testosterone, and estradiol) were measured by radioimmunoassay. Clear-cut temporal variations in plasma steroid levels were observed, which correlated with the ovarian cycle. Testosterone and estradiol-17 beta levels were low (100-200 pg/ml plasma) in early pregnancy when ovarian follicles were small but increased to about 10 ng/ml as follicles grew and gestation progressed. By contrast, plasma progesterone was high (2-6 ng/ml) in early to mid-pregnancy when corpora lutea were active and declined toward term (less than 1.0 ng/ml).
怀孕的雌性白斑角鲨在缅因州和马萨诸塞州海岸外被捕，对其肝脏、性腺和生殖道进行了形态测量和重量分析，并与繁殖周期进行了关联。采集了血浆样本，通过放射免疫分析法测量循环类固醇（孕酮、睾酮和雌二醇）。观察到血浆类固醇水平有明显的时间变化，这与卵巢周期相关。在妊娠早期，当卵巢卵泡较小时，睾酮和雌二醇-17β水平较低（血浆中为100 - 200 pg/ml），但随着卵泡生长和妊娠进展，其水平升高至约10 ng/ml。相比之下，在妊娠早期至中期，当黄体活跃时，血浆孕酮水平较高（2 - 6 ng/ml），并在足月时下降（低于1.0 ng/ml）。
