Gorodetskiĭ S I, Kaledin A S
Genetika. 1987 Apr;23(4):596-604.
Nucleotide sequence of the cloned DNA complementary to cow kappa-casein mRNA was determined. The complete sequence is composed of 854 bases and includes 60 bases of 5'-noncoding region, 570 bases of the coding region and 209 bases of 3'-noncoding region without poly(A). The cloned sequence codes for kappa-casein, the genetic variant B2. Several restriction sites were defined that permitted the identification of genetic variants and size polymorphism of restriction fragments in the structural region of the gene. Upon the comparative analysis of kappa-casein mRNAs of cow and rat, it was shown that, apart from functionally important regions in the coding sequence, high homology is characteristic of the 5'- and 3'-noncoding regions.
测定了与牛κ-酪蛋白mRNA互补的克隆DNA的核苷酸序列。完整序列由854个碱基组成，包括5′非编码区的60个碱基、编码区的570个碱基和不含poly(A)的3′非编码区的209个碱基。克隆序列编码κ-酪蛋白的遗传变体B2。确定了几个限制酶切位点，可用于鉴定该基因结构区域中遗传变体和限制片段的大小多态性。通过对牛和大鼠κ-酪蛋白mRNA的比较分析表明，除了编码序列中功能重要的区域外，5′和3′非编码区也具有高度同源性。
