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基于微流控的食源性病原体和病毒检测的适配体传感器:综述

Aptasensor Based on Microfluidic for Foodborne Pathogenic Bacteria and Virus Detection: A Review.

机构信息

An Independent Researcher, Ex Research Scholar at National Institute of Technology Durgapur, Durgapur, India.

Medical Laboratory Techniques Department, Al-Farahidi University, Baghdad, Iraq.

出版信息

Crit Rev Anal Chem. 2024;54(4):872-881. doi: 10.1080/10408347.2022.2099222. Epub 2022 Jul 13.

Abstract

In today's world, which is entangled with numerous foodborne pathogenic bacteria and viruses, it appears to be essential to rethink detection methods of these due to the importance of food safety in our lives. The vast majority of detection methods for foodborne pathogenic bacteria and viruses have suffered from sensitivity and selectivity due to the small size of these pathogens. Besides, these types of sensing approaches can improve on-site detection platforms in the fields of food safety. In recent, microfluidics systems as new emerging types of portable sensing approaches can introduce efficient and simple biodevice by integration with several analytical methods such as electrochemical, optical and colorimetric techniques. Additionally, taking advantage of aptamer as a selective bioreceptor in the sensing of microfluidics system has provided selective, sensitive, portable and affordable sensing approaches. Furthermore, some papers use increased data transferability ability and computational power of these sensing platforms by exploiting smartphones. In this review, we attempted to provide an overview of the current state of the recent aptasensor based on microfluidic for screening of foodborne pathogenic bacteria and viruses. Working strategies, benefits and disadvantages of these sensing approaches are briefly discussed.

摘要

在当今世界,由于食品安全对我们生活的重要性,我们似乎有必要重新思考这些食源性致病菌和病毒的检测方法,因为当今世界充斥着大量的食源性致病菌和病毒。由于这些病原体的体积小,绝大多数食源性致病菌和病毒的检测方法都存在灵敏度和选择性方面的问题。此外,这些类型的传感方法可以改善食品安全领域的现场检测平台。最近,微流控系统作为新型便携式传感方法,可以通过与电化学、光学和比色等多种分析方法集成,引入高效、简单的生物器件。此外,利用适体作为微流控系统传感中的选择性生物受体,提供了选择性、灵敏性、便携性和经济性的传感方法。此外,一些论文通过利用智能手机提高了这些传感平台的数据可转移性和计算能力。在这篇综述中,我们试图概述基于微流控的最近用于筛选食源性致病菌和病毒的适体传感器的最新研究进展。简要讨论了这些传感方法的工作策略、优点和缺点。

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