Department of Anatomy, Faculty of Medicine, Tekirdag Namik Kemal University, Tekirdağ, Turkey.
Department of Anatomy, Faculty of Medicine, Eskisehir Osmangazi University, Eskişehir, Turkey.
Int J Dev Neurosci. 2022 Nov;82(7):606-614. doi: 10.1002/jdn.10213. Epub 2022 Jul 21.
In this study, 32 male Sprague-Dawley rats (8 for each group) were used in total to examine the effects of valproic acid on rat cerebellum. It was determined that the experimental group received valproic acid (600 mg/kg) on embryonic day 15 and postnatal day 11, whereas the control group was treated with saline on the same days. Moreover, on the postnatal 30th day, the cerebellums of all pups were removed and prepared for light and electron microscopy. The numerical density of granule cells in the cerebellum of experimental groups of rats increased, whereas the numerical density of Purkinje cells decreased. Furthermore, the granule cells had a smaller mean nuclear diameter in one of the experimental groups, while the Purkinje cells had in both experimental groups than those in the comparison group. Thus, the numerical density of synaptic disks and their mean diameter in the cerebellar granular layer of experimental groups were significantly decreased compared to the corresponding controls; also, the synapse-to-neurons ratio, a parameter indicating interneural connectivity, was the same. Consequently, it was seen that valproic acid administration to pups in prenatal or early postnatal days causes changes in number of neurons and synapses in the cerebellum of rats.
本研究使用 32 只雄性 Sprague-Dawley 大鼠（每组 8 只），观察丙戊酸对大鼠小脑的影响。实验组于胚胎第 15 天和出生后第 11 天给予丙戊酸（600mg/kg），对照组在相同日期给予生理盐水。此外，在出生后第 30 天，所有幼鼠的小脑被取出并准备用于光镜和电镜检查。实验组大鼠小脑颗粒细胞的数值密度增加，而浦肯野细胞的数值密度降低。此外，实验组中的一个组的颗粒细胞核的平均直径较小，而两个实验组的浦肯野细胞的平均直径都比对照组小。因此，实验组小脑颗粒层的突触盘的数值密度及其平均直径与相应的对照组相比显著降低；此外，突触-神经元比，一个表示神经元间连接的参数，是相同的。因此，可见在产前或出生后早期给予幼鼠丙戊酸会导致大鼠小脑神经元和突触数量的变化。
