Ayuob Nasra, Shaker Soad Ali, Bakhshwin Abdulaziz, Alsaggaf Samar, Helal Ghada, Hamed Shereen
Department of Medical Histology, Faculty of Medicine, Damietta University, Damietta, Egypt.
Yousef Abdullatif Jameel, Chair of Prophetic Medical Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
J Microsc Ultrastruct. 2021 Jan 29;10(2):55-62. doi: 10.4103/jmau.jmau_93_20. eCollection 2022 Apr-Jun.
Statins are among the first line of pharmacological treatment of lipid disorders and lowering serum cholesterol, but they have many side effects.
The study aim was to evaluate the role of raisins in protecting the thyroid function and structure in a rat model of hypercholesterolemia, through biochemical and histopathological investigation.
Thirty male rats were randomly divided into three groups ( = 10 each) of albino rats included the control, high cholesterol diet (HCD)-fed for 13 weeks and HCD plus Raisins were included in this study. Blood levels of glucose, insulin, cholesterol, lipids, thyroid-stimulating hormone (TSH), T3, T4, oxidants/anti-oxidants were assessed. Thyroid gland was processed and examined histopathologically using light and electron microscopy.
Feeding HCD resulted in hypercholesterolemia in rats after 13 weeks as evidence by lipid profile. Ingestion of raisins along with HCD resulted in a significant ( < 0.001) decrease in the levels of insulin, blood glucose, thyroxine (T4) and malondialdehyde (MDA), while the levels of TSH, T3 and total anti-oxidant capacity significantly ( < 0.001) elevated. Raisins histologically alleviated the HCD-induced structural changes in the thyroid glands that included degenerated mitochondria and increased lipid droplets in the cytoplasm.
Simultaneous administration of raisins along with HCD, administrated for a short time, could modulate the negative effect on thyroid gland structure and function.
他汀类药物是脂质紊乱和降低血清胆固醇的一线药物治疗，但它们有许多副作用。
本研究旨在通过生化和组织病理学研究，评估葡萄干在高胆固醇血症大鼠模型中对甲状腺功能和结构的保护作用。
30只雄性大鼠随机分为三组（每组10只）白化大鼠，包括对照组、喂食高胆固醇饮食（HCD）13周的组和本研究中的HCD加葡萄干组。评估血糖、胰岛素、胆固醇、脂质、促甲状腺激素（TSH）、T3、T4、氧化剂/抗氧化剂的血液水平。对甲状腺进行处理，并使用光学和电子显微镜进行组织病理学检查。
喂食HCD 13周后，大鼠出现高胆固醇血症，血脂谱可证明这一点。与HCD一起摄入葡萄干导致胰岛素、血糖、甲状腺素（T4）和丙二醛（MDA）水平显著降低（<0.001），而TSH、T3和总抗氧化能力水平显著升高（<0.001）。葡萄干在组织学上减轻了HCD诱导的甲状腺结构变化，包括线粒体退化和细胞质中脂滴增加。
短时间内将葡萄干与HCD同时给药，可以调节对甲状腺结构和功能的负面影响。
