Yoshida Toshinori, Kobayashi Mio, Uomoto Suzuka, Ohshima Kanami, Hara Emika, Katoh Yoshitaka, Takahashi Naofumi, Harada Takanori, Usui Tatsuya, Elbadawy Mohamed, Shibutani Makoto
Laboratory of Veterinary Pathology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Cooperative Division of Veterinary Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
J Toxicol Pathol. 2022 Jul;35(3):225-235. doi: 10.1293/tox.2022-0017. Epub 2022 May 23.
The development of toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin-stained specimens. This was done while considering the usefulness and limitations of studies for toxicologic pathology assessment.
使用培养细胞的毒性评估方法的发展，在促进动物实验中的动物福利方面越来越受欢迎。在此，我们简要讨论使用人源和大鼠源肝细胞进行肝毒性评估的现状；我们重点关注近年来得到广泛研究的肝脏类器官方法，并讨论毒理病理学家如何利用他们的知识和经验，为药物、农药和化学品的化学肝毒性评估方法的发展做出贡献。我们还提出，当在苏木精和伊红染色的标本中观察肝脏类器官时，毒理病理学家应如何评估类器官中未分化和分化细胞的假定分布的毒性。这样做的同时考虑了毒理病理学评估研究的有用性和局限性。
