Onishi Shinichi, Murai Atsuko, Kito Aki, Kawashima Yuka, Ohmori Yusuke, Kato Atsuhiko
Translational Research Division, Chugai Pharmaceutical Co., Ltd., 1-135 Komakado, Gotemba, Shizuoka 412-8513, Japan.
Research Division, Chugai Pharmaceutical Co., Ltd., 1-135 Komakado, Gotemba, Shizuoka 412-8513, Japan.
J Toxicol Pathol. 2022 Jul;35(3):275-279. doi: 10.1293/tox.2022-0008. Epub 2022 May 7.
Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification.
前庭器官由位于椭圆囊和球囊内的位觉斑以及半规管及其壶腹组成。目前尚无关于包括位觉斑或半规管在内的前庭器官标本制备方法的报道。在本研究中，我们研究了用于组织病理学检查的高度可重复的前庭器官标本制备方法。我们建立了一种方法，使研究人员能够观察椭圆囊和球囊，包括耳石、半规管壶腹以及部分半规管。这种高度可重复的方法对于对因药物毒性和基因改造导致平衡异常症状的小鼠进行组织病理学分析很有用。
