Kimber I, Sparshott S M, Bell E B, Ford W L
Immunology. 1987 Apr;60(4):585-91.
Lymphocytes from the thoracic duct (TDL) were incubated with interferon (IFN) prior to i.v. injection into syngeneic or allogeneic recipient rats. The effect of IFN treatment on the ability of lymphocytes to migrate was studied using 'standard' TDL collected overnight at 4 degrees or an 'optimal' collection of passaged TDL which recirculate with an accelerated tempo (Smith & Ford, 1983). Interferon treatment resulted in an increase in early (30 min) localization of both standard and optimal TDL into lymph nodes. Entry of standard IFN-treated TDL was increased by 91% and 54% in cervical and mesenteric lymph nodes, respectively; increases of 50% and 22% in the same lymph nodes were recorded for optimal IFN-treated TDL. Enhanced entry of standard TDL was contrasted with a reduced ability of IFN-treated TDL to migrate out of lymph nodes; there was a reduced output into the thoracic duct and a surplus of IFN-treated lymphocytes in cervical lymph nodes despite 24 hr continuous thoracic duct drainage. Incubation with interferon did not, however, alter the ability of optimal TDL to reach the thoracic duct rapidly after injection. Allogeneic lymphocytes, which are eliminated soon after injection by an NK-like cytotoxicity, a phenomenon termed ALC, were unaffected by incubation with interferon, thus IFN-treated allogeneic lymphocytes were killed after i.v. injection as rapidly as untreated cells.
将来自胸导管的淋巴细胞（TDL）在静脉注射到同基因或异基因受体大鼠之前，先用干扰素（IFN）进行孵育。使用在4℃下过夜收集的“标准”TDL或经传代的TDL的“最佳”收集物（其以加速的节奏再循环，Smith和Ford，1983）来研究IFN处理对淋巴细胞迁移能力的影响。干扰素处理导致标准TDL和最佳TDL在早期（30分钟）在淋巴结中的定位增加。经标准IFN处理的TDL在颈淋巴结和肠系膜淋巴结中的进入量分别增加了91%和54%；经最佳IFN处理的TDL在相同淋巴结中的增加量分别为50%和22%。标准TDL进入的增强与经IFN处理的TDL从淋巴结中迁移出来的能力降低形成对比；尽管胸导管连续引流24小时，但经IFN处理的淋巴细胞进入胸导管的量减少，并且颈淋巴结中有多余的经IFN处理的淋巴细胞。然而，与干扰素孵育并没有改变最佳TDL在注射后迅速到达胸导管的能力。同种异体淋巴细胞在注射后很快会被一种称为ALC的自然杀伤样细胞毒性作用清除，它不受与干扰素孵育的影响，因此经IFN处理的同种异体淋巴细胞在静脉注射后与未处理的细胞一样迅速被杀死。
