Westermann J, Persin S, Matyas J, van der Meide P, Pabst R
Center of Anatomy, Medical School of Hannover, Federal Republic of Germany.
J Immunol. 1993 May 1;150(9):3843-52.
Thoracic duct lymphocytes (TDL) continuously patrol through the body, facilitating immune responses at most sites. IFN-gamma might regulate immune responses by influencing the migration of TDL. Therefore, it was investigated in vivo whether IFN-gamma affects the migration of thoracic duct B, T, CD4+, and CD8+ lymphocytes from blood to lymph. Labeled TDL were injected i.v. into rats continuously receiving IFN-gamma via a central venous catheter. The numbers of B, T, CD4+, and CD8+ lymphocytes were determined in blood and thoracic duct lymph for 120 h. IFN-gamma increased the disappearance of TDL from the blood to a similar extent in all subsets. In contrast, the reappearance of B and T lymphocyte subsets in the lymph was decreased: B lymphocytes were affected significantly more than T lymphocytes, whereas CD4+ and CD8+ lymphocytes were affected to a similar extent. Our study suggests that differential retention within the tissue rather than preferential immigration into the tissue creates a microenvironment with a distinct composition of lymphocyte subsets.
胸导管淋巴细胞（TDL）持续在体内循环，促进大多数部位的免疫反应。干扰素-γ可能通过影响TDL的迁移来调节免疫反应。因此，我们在体内研究了干扰素-γ是否会影响胸导管B淋巴细胞、T淋巴细胞、CD4⁺淋巴细胞和CD8⁺淋巴细胞从血液迁移至淋巴。将标记的TDL静脉注射到通过中心静脉导管持续接受干扰素-γ的大鼠体内。在120小时内测定血液和胸导管淋巴液中B淋巴细胞、T淋巴细胞、CD4⁺淋巴细胞和CD8⁺淋巴细胞的数量。干扰素-γ使所有亚群中TDL从血液中的消失程度增加到相似水平。相比之下，淋巴液中B淋巴细胞和T淋巴细胞亚群的重新出现减少：B淋巴细胞受到的影响明显大于T淋巴细胞，而CD4⁺淋巴细胞和CD8⁺淋巴细胞受到的影响程度相似。我们的研究表明，组织内的差异滞留而非优先迁入组织，造就了具有独特淋巴细胞亚群组成的微环境。
