Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, Avcilar, Istanbul, Turkey.
J Biochem Mol Toxicol. 2022 Oct;36(10):e23169. doi: 10.1002/jbt.23169. Epub 2022 Jul 14.
Pentylenetetrazole (PTZ) is preferred for experimental epilepsy induction. PTZ damages brain and other organs by elevating oxidative substances. Vitamin U (Vit U) is sulfur derivative substance that proved to be an excellent antioxidant. The current study was intended to determine the protective role of Vit U on PTZ-induced brain damage. Male Sprague-Dawley rats were separated into four groups. The Control group (Group I), was given saline for 7 days intraperitoneally (i.p); Vit U (Group II) was given as 50 mg/kg/day for 7 days by gavage; PTZ was injected into animals (Group III) at a single dose of 60 mg/kg, by i.p; PTZ + Vit U group (Group IV) was administered PTZ and Vit U in same dose and time as aforementioned. After the experiment was terminated, brain tissues were taken for the preparation of homogenates. In the PTZ group, glutathione and lipid peroxidation levels, alkaline phosphatase, myeloperoxidase, xanthine oxidase, acetylcholine esterase, antioxidant enzyme activities, total oxidant status, oxidative stress index, reactive oxygen species, and nitric oxide levels were increased. However, total antioxidant capacity was decreased in the PTZ group. Vit U ameliorated these effects in the PTZ-induced brain damage. Consequently, we can suggest that Vit U protected brain tissue via its antioxidant feature against PTZ kindling epilepsy.
戊四氮（PTZ）是实验性癫痫诱导的首选药物。PTZ 通过升高氧化物质对大脑和其他器官造成损害。维生素 U（Vit U）是一种硫衍生物物质，已被证明是一种出色的抗氧化剂。本研究旨在确定 Vit U 对 PTZ 诱导的脑损伤的保护作用。雄性 Sprague-Dawley 大鼠分为四组。对照组（I 组）连续 7 天腹腔内给予生理盐水；Vit U 组（II 组）连续 7 天灌胃给予 50mg/kg/天；PTZ 组（III 组）单次腹腔内注射 60mg/kg；PTZ+Vit U 组（IV 组）以与上述相同的剂量和时间给予 PTZ 和 Vit U。实验结束后，取脑组织制备匀浆。在 PTZ 组，谷胱甘肽和脂质过氧化水平、碱性磷酸酶、髓过氧化物酶、黄嘌呤氧化酶、乙酰胆碱酯酶、抗氧化酶活性、总氧化剂状态、氧化应激指数、活性氧和一氧化氮水平升高。然而，PTZ 组的总抗氧化能力下降。Vit U 改善了 PTZ 诱导的脑损伤中的这些作用。因此，我们可以认为 Vit U 通过其抗氧化特性保护脑组织免受 PTZ 点燃性癫痫的影响。
