Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Izmir Katip Çelebi University, 35620, Izmir, Turkey.
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, 35040, Izmir, Turkey.
Curr Microbiol. 2022 Jul 14;79(9):256. doi: 10.1007/s00284-022-02943-0.
Acinetobacter baumannii is a significant nosocomial pathogen, with its biofilm forming capacity playing an important role in its pathogenicity. The fast and reliable detection of the biofilm formation and measurement of antibiofilm activity of various molecules are critical for combating A. baumannii infections. In this study, we aimed to detect biofilm formation by real time cell analyses (RTCA) method in clinical A. baumannii isolates and to investigate antibiofilm activities of tigecycline (TGC), N-acetylcysteine (NAC), and acetylsalicylic acid (ASA). The effect of the tested drugs on expressions of biofilm-related genes bap and csuE in clinical A. baumannii strains was also analyzed by real time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Biofilm forming capacities for strong and weak biofilm producer A. baumannii strains were detected within 10 h by RTCA method (P < 0.05). We also observed that sub-minimum inhibitory concentrations of NAC + TGC and ASA + TGC combinations could significantly reduce biofilm formation and expression of biofilm-related genes in A. baumanii strains. No statistically significant activity of the tested drugs was detected against mature biofilms of the bacterial strains with the RTCA method. These results suggest that reproducible results on biofilm production capacity of A. baumannii strains and antibiofilm activities of various compounds can be obtained in a short time using RTCA method. Therefore, RTCA method seems to be a beneficial technique for biofilm detection and can help in combating A. baumannii infections by giving health providers the opportunity of implementing antibiofilm treatment strategies in a timely manner.
鲍曼不动杆菌是一种重要的医院获得性病原体，其生物膜形成能力在其致病性中起着重要作用。快速可靠地检测生物膜形成并测量各种分子的抗生物膜活性对于对抗鲍曼不动杆菌感染至关重要。在这项研究中，我们旨在通过实时细胞分析（RTCA）方法检测临床鲍曼不动杆菌分离株的生物膜形成，并研究替加环素（TGC）、N-乙酰半胱氨酸（NAC）和乙酰水杨酸（ASA）的抗生物膜活性。还通过实时定量逆转录聚合酶链反应（RT-qPCR）分析了测试药物对临床鲍曼不动杆菌菌株中生物膜相关基因 bap 和 csuE 表达的影响。RTCA 方法在 10 小时内检测到强和弱生物膜生成鲍曼不动杆菌菌株的生物膜形成能力（P<0.05）。我们还观察到 NAC+TGC 和 ASA+TGC 联合使用亚最小抑菌浓度可显著降低鲍曼不动杆菌菌株生物膜形成和生物膜相关基因的表达。用 RTCA 方法未检测到测试药物对细菌菌株成熟生物膜的统计学显著活性。这些结果表明，RTCA 方法可在短时间内获得鲍曼不动杆菌菌株生物膜形成能力和各种化合物抗生物膜活性的可重复结果。因此，RTCA 方法似乎是一种用于生物膜检测的有益技术，通过为医疗保健提供者提供及时实施抗生物膜治疗策略的机会，有助于对抗鲍曼不动杆菌感染。
