粪便样本中 Strongyloides stercoralis DNA 的检测对 2 型糖尿病患者的重要性。
Importance of detection of Strongyloides stercoralis DNA in fecal samples from patients with type 2 diabetes mellitus.
机构信息
Laboratório de Parasitologia, Universidade Federal de Jataí, Jataí, GO, Brazil.
Laboratório de Investigação Médica, Hospital de Clínicase Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, Brazil.
出版信息
Clinics (Sao Paulo). 2022 Jul 11;77:100060. doi: 10.1016/j.clinsp.2022.100060. eCollection 2022.
OBJECTIVE
The association between diabetes and Strongyloides infection remains controversial. This study aimed to detect Strongyloides stercoralis DNA in the feces of patients with Diabetes Mellitus type 2 (DM2).
METHODS
Fecal samples were analyzed via the Lutz, Rugai, and agar plate culture methods. PCR amplification was performed using two targets (PCR-genus and PCR-species) located on the S. stercoralis 18S ribosomal.
RESULTS
The positivity for S. stercoralis using parasitological methods was 1.1%. PCR-genus (14.13%) demonstrated a higher positivity than PCR-species (9.78%).
CONCLUSION
The results confirm the greater positivity of the molecular diagnosis in relation to parasitological methods, reinforcing its use as an additional tool for the diagnosis of S. stercoralis infection in patients with DM2 living in endemic areas for this helminthiasis.
目的
糖尿病与旋毛虫感染之间的关系仍存在争议。本研究旨在检测 2 型糖尿病（DM2）患者粪便中的粪类圆线虫 DNA。
方法
采用 Lutz、Rugai 和琼脂平板培养方法对粪便样本进行分析。使用位于 S. stercoralis 18S 核糖体上的两个靶标（PCR-属和 PCR-种）进行 PCR 扩增。
结果
寄生虫学方法检测到的粪类圆线虫阳性率为 1.1%。PCR-属（14.13%）的阳性率高于 PCR-种（9.78%）。
结论
这些结果证实了分子诊断相对于寄生虫学方法具有更高的阳性率，这加强了其作为诊断 DM2 患者中流行地区粪类圆线虫感染的额外工具的使用。
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