College of Environmental Sciences, Sichuan Agricultural University, Wenjiang 611130, China; Chengdu Agricultural College, Wenjiang 611130, China.
College of Environmental Sciences, Sichuan Agricultural University, Wenjiang 611130, China; Sichuan Provincial Key Laboratory of Soil Environmental Protection, Wenjiang 611130, China.
Ecotoxicol Environ Saf. 2022 Sep 1;242:113862. doi: 10.1016/j.ecoenv.2022.113862. Epub 2022 Jul 11.
Toxic metal contamination causes a great threat to soil ecosystem and human health. Soil washing is a fast practice for removing metals, but its influences on microbial diversity and the stability of soil ecosystem remain unknown. In this study, ethylenediaminetetraacetic acid (EDTA), citric acid (CA), and fermented pineapple peel residue (FPP) were used as representatives of chelates, low molecular organic acids and biological materials to wash Pb-polluted soils, and their impacts on microbial community were investigated. Washing with these agents effectively removed Pb, but altered microbial community structure. After washing with EDTA, CA, and FPP, 3-8 bacterial phyla and 1 fungal phylum greatly increased, while 7-20 bacterial and 0-6 fungal phyla severely decreased or even disappeared. The alterations of different microbiomes were closely related to soil metal fractions. The labile metal fraction had negative effects on most bacteria and fungi, but also showed positive influences on Actinobacteria, Patescibacteria, and Fusobacteria. The moderately stable and stable fractions were nontoxic to the most microbes, but still harmful to Patescibacteria and Deinococcus-Thermus. These findings provide new insights for the effects of soil washing remediation and toxicity of metal fractions on the microbiomes with different abundance.
有毒金属污染对土壤生态系统和人类健康构成了巨大威胁。土壤洗脱是一种快速去除金属的方法，但它对微生物多样性和土壤生态系统稳定性的影响尚不清楚。在这项研究中，乙二胺四乙酸（EDTA）、柠檬酸（CA）和发酵菠萝皮残渣（FPP）被用作螯合剂、低分子有机酸和生物材料的代表，用于洗脱 Pb 污染土壤，并研究了它们对微生物群落的影响。这些试剂的洗脱有效地去除了 Pb，但改变了微生物群落结构。用 EDTA、CA 和 FPP 洗脱后，3-8 个细菌门和 1 个真菌门大量增加，而 7-20 个细菌门和 0-6 个真菌门严重减少甚至消失。不同微生物组的变化与土壤金属形态密切相关。可利用金属形态对大多数细菌和真菌都有负面影响，但对放线菌、巴氏菌门和厚壁菌门也有积极影响。中等稳定和稳定的形态对大多数微生物没有毒性，但对巴氏菌门和 Deinococcus-Thermus 仍有危害。这些发现为土壤洗脱修复的影响以及金属形态对不同丰度微生物组的毒性提供了新的见解。
