Hamprecht K, Handgretinger R, Voetsch W, Anderer F A
Int J Immunopharmacol. 1987;9(2):199-209. doi: 10.1016/0192-0561(87)90095-6.
Viscum album extracts (Iscador) were investigated for their potency to influence NK cytotoxicity in vitro. In vitro short term cytotoxicity assays (4 h) with human peripheral mononuclear cells (PMNC) and human K 562 tumor cells showed a drastic enhancement of NK cytotoxicity in the presence of V. album extracts. The presence of the V. album components during tumor cell lysis was essential since preincubation of PMNC with V. album extract followed by thorough washing did not lead to enhancement of NK cytotoxicity. One responding effector cell was identified as a member of the large granular lymphocyte (LGL) family carrying both Leu 7 and Leu 11 surface markers. Furthermore, monocytes depleted of LGL, but not differentiated macrophages, showed a weak enhancement of their cytolytic activity in the presence of V. album extract. Fractionation of V. album extracts revealed two active fractions one (C1) with about 3-4000 D and the other (C2) less than 1000 D. Both components enhanced NK cytotoxicity of LGL (Leu 7+, Leu 11+) as well as of monocytes showing enhancing effects also against moderately NK-sensitive tumor cell lines.
研究了欧洲槲寄生提取物（Iscador）在体外影响自然杀伤细胞（NK）细胞毒性的能力。用人外周血单个核细胞（PMNC）和人K562肿瘤细胞进行的体外短期细胞毒性试验（4小时）表明，在欧洲槲寄生提取物存在的情况下，NK细胞毒性显著增强。肿瘤细胞裂解过程中欧洲槲寄生成分的存在至关重要，因为用欧洲槲寄生提取物预孵育PMNC后彻底洗涤并不会导致NK细胞毒性增强。一个反应性效应细胞被鉴定为携带Leu 7和Leu 11表面标志物的大颗粒淋巴细胞（LGL）家族成员。此外，去除LGL但未分化为巨噬细胞的单核细胞在欧洲槲寄生提取物存在的情况下，其溶细胞活性有微弱增强。欧洲槲寄生提取物的分级分离显示出两个活性组分，一个（C1）分子量约为3 - 4000 D，另一个（C2）分子量小于1000 D。这两种组分均增强了LGL（Leu 7 +，Leu 11 +）以及单核细胞的NK细胞毒性，对中度NK敏感的肿瘤细胞系也显示出增强作用。
