Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University), Chongqing, China.
Laboratory of Human Disease and Immunotherapies, West China Hospital, Sichuan University, Chengdu, China.
Methods Mol Biol. 2022;2525:21-34. doi: 10.1007/978-1-0716-2473-9_3.
Reactive oxygen species (ROS) play a key role in various physiological and pathological processes. Abnormally elevated ROS levels are generally related to the pathogenesis of inflammatory diseases and tumors. Real-time imaging and quantification of ROS can not only provide new insight into mechanistic understanding of diseases associated with ROS but also facilitate high-throughput and high-content drug screening for these diseases. Here, the present protocol introduces ROS-responsive and self-illuminating nanoparticles with chemiluminescence (CL) and fluorescence (FL) properties that can serve as an effective nanoprobe for imaging of pathophysiology, including inflammation and tumor.
活性氧（ROS）在各种生理和病理过程中发挥着关键作用。ROS 水平异常升高通常与炎症性疾病和肿瘤的发病机制有关。ROS 的实时成像和定量不仅可以为与 ROS 相关疾病的机制理解提供新的见解，还可以促进这些疾病的高通量和高内涵药物筛选。这里，本方案介绍了具有化学发光（CL）和荧光（FL）性质的 ROS 响应型自发光纳米粒子，可作为用于成像病理生理学（包括炎症和肿瘤）的有效纳米探针。
