Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.
Trop Biomed. 2022 Jun 1;39(2):170-178. doi: 10.47665/tb.39.2.002.
Cestode infections is widely reported in rodents, however species identification remains problematic due to the genetic or interspecies variation. Therefore, this study was aimed to verify the Cyclophyllidean parasites recovered from wild rats captured from different forest types using molecular based methods. Maximum-likelihood (ML) and neighbour-joining (NJ) trees were constructed inferred from 18 small subunit nuclear ribosomal RNA gene (18SrDNA) and mitochondrial cytochrome c oxidase subunit one gene (COX1) sequences of cestode worms recovered from 124 individuals from four rat species. Sequences obtained from both Hymenolepis diminuta and Hydatigera parva represents the first records in Malaysia. All the sequences were successfully amplified with product with total length of 205 and 1202 base pairs (bp), respectively. Three cestode species from the Family Hymenolepididae (Hymenolepis diminuta) and Family Taeniidae (Hydatigera parva; Hydatigera taeniaeformis) were successfully characterized using phylogenetic analyses and haplotype networking. Phylogenetic analysis showed that H. diminuta, Hydatigera parva (Hy. parva) and Hydatigera taeniaeformis (Hy. taeniaeformis) formed its own monophyletic clade in 18SrDNA analyses. Results also showed that Hy. taeniaeformis shared the same haplotype group with Hy. taeniaeformis from China (COX1) and linked with Hy. taeniaeformis from Japan (18SrDNA) while the Malaysian H. diminuta clearly formed a separate haplotype and networked with other regions. The Malaysian Hy. parva isolation, on the other hand, appeared to be genetically distinct from the European Hy. parva (Spain) strain, but closely linked to the local isolates. Molecular methods employed successfully improved in the detection of complex species in this group. The findings showed that molecular data can be useful to deeply study intra-specific variation in other cestode worms.
带绦虫感染在啮齿动物中广泛报道，但由于遗传或种间变异，物种鉴定仍然存在问题。因此，本研究旨在使用分子方法验证从四种不同森林类型捕获的野生大鼠中回收的环绦虫寄生虫。使用来自 124 只个体的 18 小亚基核核糖体 RNA 基因（18SrDNA）和线粒体细胞色素 c 氧化酶亚基 I 基因（COX1）序列的最大似然（ML）和邻接法（NJ）树从 124 只个体中构建了推测的带绦虫蠕虫。从 Hymenolepis diminuta 和 Hydatigera parva 获得的序列代表马来西亚的首次记录。所有序列均成功扩增，产物全长分别为 205 和 1202 个碱基对（bp）。使用系统发育分析和单倍型网络成功鉴定了来自 Hymenolepididae 科（Hymenolepis diminuta）和 Taeniidae 科（Hydatigera parva；Hydatigera taeniaeformis）的三种带绦虫物种。系统发育分析表明，H. diminuta、Hydatigera parva（Hy. parva）和 Hydatigera taeniaeformis（Hy. taeniaeformis）在 18SrDNA 分析中形成了自己的单系分支。结果还表明，Hy. taeniaeformis 与中国的 Hy. taeniaeformis（COX1）共享相同的单倍型组，并与日本的 Hy. taeniaeformis（18SrDNA）相连，而马来西亚的 H. diminuta 则明显形成了一个单独的单倍型，并与其他地区的单倍型相连。另一方面，马来西亚的 Hy. parva 分离株在遗传上与欧洲的 Hy. parva（西班牙）株不同，但与当地分离株密切相关。所采用的分子方法成功提高了该组中复杂物种的检测能力。研究结果表明，分子数据可用于深入研究其他带绦虫蠕虫的种内变异。
