冻存人外周血单个核细胞（PBMCs）中线粒体呼吸的测量。
Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs).
机构信息
Division of Development of Mental Functions, Research Center for Child Mental Development, University of Fukui, Fukui, Japan.
United Graduate School of Child Development, Osaka University, Osaka, Japan.
出版信息
Methods Mol Biol. 2022;2511:321-332. doi: 10.1007/978-1-0716-2395-4_24.
Inflammatory diseases caused by infectious agents such as the SARS-CoV-2 virus can lead to impaired reductive-oxidative (REDOX) balance and disrupted mitochondrial function. Peripheral blood mononuclear cells (PBMCs) provide a useful model for studying the effects of inflammatory diseases on mitochondrial function but can be limited by the need to store these cells by cryopreservation prior to assay. Here, we describe a method for improving and determining PBMC viability with normalization of values to number of living cells. The approach can be applied not only to PBMC samples derived from patients with diseases marked by an altered inflammatory response such as viral infections.
由 SARS-CoV-2 病毒等感染因子引起的炎症性疾病可导致还原性氧化（REDOX）平衡受损和线粒体功能障碍。外周血单核细胞（PBMC）为研究炎症性疾病对线粒体功能的影响提供了一个有用的模型，但由于需要在测定前通过冷冻保存来储存这些细胞，因此存在一定的局限性。在这里，我们描述了一种改进和确定 PBMC 活力的方法，并将其值归一化为活细胞数量。该方法不仅可以应用于源自炎症反应改变的疾病（如病毒感染）患者的 PBMC 样本。
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