National Engineering Research Center for Biomaterials, Sichuan University, No. 29 Wangjiang Road, Chengdu 610064, P. R. China.
Department of Cardiovascular Surgery, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu 610041 P.R. China.
ACS Biomater Sci Eng. 2022 Aug 8;8(8):3399-3410. doi: 10.1021/acsbiomaterials.2c00367. Epub 2022 Jul 15.
Bioprosthetic heart valves (BHVs) are widely used in clinic, but they still have problems of calcification, thrombogenicity, and cytotoxicity. The reported techniques based on glutaraldehyde (Glut) crosslinking have difficulty in solving these problems simultaneously. In this study, we grafted Glut-crosslinked porcine pericardium (GA) with hyaluronic acid (HA) by radical copolymerization to improve its anticalcification and antithrombotic properties. Partially methacrylated poly-ε-lysine was used to introduce methacryl groups into GA. Then, HA-grafted porcine pericardium (GA-HA) was obtained by radical copolymerization. Rat's subcutaneous implantation results showed that the calcium content of GA-HA was significantly lower than that of GA (37 ± 29 μg/mg vs 188 ± 7 μg/mg), and the platelets adhering to the surface of GA-HA decreased by approximately 41% compared with GA. In conclusion, grafting porcine pericardium with HA by copolymerization might be feasible to improve the anticalcification and antithrombotic properties of BHVs.
生物瓣（BHVs）在临床上被广泛应用，但仍存在钙化、血栓形成和细胞毒性等问题。基于戊二醛（Glut）交联的报道技术难以同时解决这些问题。在本研究中，我们通过自由基共聚将戊二醛交联的猪心包（GA）接枝到透明质酸（HA）上，以改善其抗钙化和抗血栓形成特性。部分甲基丙烯酰化聚赖氨酸用于将甲基丙烯酰基引入 GA。然后，通过自由基共聚得到接枝有透明质酸的猪心包（GA-HA）。大鼠皮下植入结果表明，GA-HA 的钙含量明显低于 GA（37±29μg/mg 比 188±7μg/mg），与 GA 相比，附着在 GA-HA 表面的血小板减少了约 41%。总之，通过共聚将 HA 接枝到猪心包上可能是改善 BHVs 抗钙化和抗血栓形成特性的一种可行方法。
